ArticleGene therapy2007
Therapeutic RNA interference of malignant melanoma by electrotransfer of small interfering RNA targeting Mitf.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Upregulation of the novel lncRNA U731166 is associated with migration, invasion and vemurafenib resistance in melanoma.Journal of cellular and molecular medicine · 2022Article
- Use of Nanoparticles in Delivery of Nucleic Acids for Melanoma Treatment.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Skin Delivery of siRNA Using Sponge Spicules in Combination with Cationic Flexible Liposomes.Molecular therapy. Nucleic acids · 2020Article
- Transdermal Delivery of siRNA through Microneedle Array.Scientific reports · 2016Article
- Article
- Microphthalmia-associated transcription factor in melanoma development and MAP-kinase pathway targeted therapy.Pigment cell & melanoma research · 2015Review
- Short-interference RNAs: becoming medicines.EXCLI journal · 2015Article
- Acid sphingomyelinase determines melanoma progression and metastatic behaviour via the microphtalmia-associated transcription factor signalling pathway.Cell death and differentiation · 2014Article
- Gene Silencing in Skin After Deposition of Self-Delivery siRNA With a Motorized Microneedle Array Device.Molecular therapy. Nucleic acids · 2013Article
- Delivery of RNAi-Based Oligonucleotides by Electropermeabilization.Pharmaceuticals (Basel, Switzerland) · 2013Article
- Gene silencing following siRNA delivery to skin via coated steel microneedles: In vitro and in vivo proof-of-concept.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Progress toward in vivo use of siRNAs-II.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Review
- Preclinical development of siRNA therapeutics for AL amyloidosis.Gene therapy · 2011Article
- Bioelectric applications for treatment of melanoma.Cancers · 2010Article
- Regulation of cytokines by small RNAs during skin inflammation.Journal of biomedical science · 2010Review
- MicroRNAs targeting mutant K-ras by electrotransfer inhibit human colorectal adenocarcinoma cell growth in vitro and in vivo.Cancer gene therapy · 2010Article
- Simultaneous suppression of MITF and BRAF V600E enhanced inhibition of melanoma cell proliferation.Cancer science · 2009Article
- Amelioration of psoriasis by anti-TNF-alpha RNAi in the xenograft transplantation model.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Allele-specific silencing of the dominant disease allele in sialuria by RNA interference.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- In vivo gene silencing in solid tumors by targeted electrically mediated siRNA delivery.Gene therapy · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microphthalmia-associated transcription factor (Mitf) is critically involved in melanin synthesis as well as differentiation of cells of the melanocytic lineage. Some earlier studies suggested that Mitf is also essential in the survival of melanoma cells, but this notion remains controversial. We synthesized short interfering RNA (siRNA) duplexes corresponding to the mitf sequence and transfected them into B16 melanoma. Lipid-mediated transfection in vitro of Mitf-specific siRNA resulted in specific downregulation of Mitf and of the tyrosinase that is a transcriptional target of Mitf. This treatment also remarkably reduced the viability of melanoma cells by inducing apoptosis. To examine the potential feasibility of RNAi therapy against melanoma, B16 cells were subcutaneously injected into syngenic mice and siRNA was transfected into the pre-established tumor by means of electroporation. The Mitf-specific siRNA drastically reduced outgrowth of subcutaneous melanoma, while nonspecific siRNA failed to affect tumor progression. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling-based analysis of tumor specimens demonstrated that the tumor cells transfected with Mitf-siRNA effectively underwent apoptosis in vivo. The present results indicate that Mitf plays important roles in melanoma survival. Intratumor electrotransfer of Mitf-specific siRNA may provide a powerful strategy for therapeutic intervention of malignant melanoma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.