ArticleMolecular biology of the cell2006
A eukaryotic capsular polysaccharide is synthesized intracellularly and secreted via exocytosis.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
115 citing papers in PubMed, 171 citations in OpenAlex.
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- Microglia sense fungal infections through capsular components from capillary-bound Cryptococcus neoformans via endothelial nucleotide signaling.PLoS biology · 2026Article
- The protein phosphatase 2C domain contributes to the pathobiological function of adenylyl cyclase inbioRxiv : the preprint server for biology · 2025Article
- The Zinc Finger Protein Zfp2 Regulates Cell-Cell Fusion and Virulence inJournal of fungi (Basel, Switzerland) · 2025Article
- Rta1 lipid transporter involved in aluminum and acid tolerance in Cryptococcus humicola.Folia microbiologica · 2025Article
- Evolutionary uniqueeLife · 2025Article
- Leveraging a new data resource to define the response of Cryptococcus neoformans to environmental signals.Genetics · 2025Article
- Virulence factors released by extracellular vesicles fromFrontiers in cellular and infection microbiology · 2025Review
- Cell wall melanin impedes growth of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
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- Host-Pathogen Interactions and Correlated Factors That Are Affected in Replicative-AgedJournal of fungi (Basel, Switzerland) · 2024Article
- Synthetic Glycans Reveal Determinants of Antibody Functional Efficacy against a Fungal Pathogen.ACS infectious diseases · 2024Article
- Loss of the putative Rab GTPase, Ypt7, impairs the virulence ofFrontiers in microbiology · 2024Article
- Phosphate availability conditions caspofungin tolerance, capsule attachment and titan cell formation inFrontiers in fungal biology · 2024Article
- More complicated than it seems: The diversity of cryptococcal glucuronoxylomannan.PLoS pathogens · 2023Article
- Synergistic antifungal interaction ofFrontiers in microbiology · 2023Article
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- A capsule-associated gene ofMicrobiology (Reading, England) · 2021Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cryptococcus neoformans, which causes fatal infection in immunocompromised individuals, has an elaborate polysaccharide capsule surrounding its cell wall. The cryptococcal capsule is the major virulence factor of this fungal organism, but its biosynthetic pathways are virtually unknown. Extracellular polysaccharides of eukaryotes may be made at the cell membrane or within the secretory pathway. To test these possibilities for cryptococcal capsule synthesis, we generated a secretion mutant in C. neoformans by mutating a Sec4/Rab8 GTPase homolog. At a restrictive temperature, the mutant displayed reduced growth and protein secretion, and accumulated approximately 100-nm vesicles in a polarized manner. These vesicles were not endocytic, as shown by their continued accumulation in the absence of polymerized actin, and could be labeled with anti-capsular antibodies as visualized by immunoelectron microscopy. These results indicate that glucuronoxylomannan, the major cryptococcal capsule polysaccharide, is trafficked within post-Golgi secretory vesicles. This strongly supports the conclusion that cryptococcal capsule is synthesized intracellularly and secreted via exocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.