ArticleApplied and environmental microbiology2006
Natural genetic transformation: A novel tool for efficient genetic engineering of the dairy bacterium Streptococcus thermophilus.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Engineering Streptococcus thermophilus for heterologous gene expression of cell envelope proteases from lactic acid bacteria.Microbial cell factories · 2026Article
- CRISPR adaptation inmSphere · 2025Article
- Advances in Genetic Transformation of Lactic Acid Bacteria: Overcoming Barriers and Enhancing Plasmid Tools.International journal of molecular sciences · 2025Review
- Use of Rgg quorum-sensing machinery to create an innovative recombinant protein expression system inMicrobiology (Reading, England) · 2024Article
- Advances in Genetic Tools and Their Application inFoods (Basel, Switzerland) · 2023Review
- Development of a Tetracycline-Inducible System for Conditional Gene Expression in Lactococcus lactis and Streptococcus thermophilus.Microbiology spectrum · 2023Article
- Expanding natural transformation to improve beneficial lactic acid bacteria.FEMS microbiology reviews · 2022Article
- NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens.Communications biology · 2022Article
- Genetic tools for the development of recombinant lactic acid bacteria.Microbial cell factories · 2021Review
- An Inventory of CiaR-Dependent Small Regulatory RNAs inFrontiers in microbiology · 2021Review
- Intensive targeting of regulatory competence genes by transposable elements in streptococci.Molecular genetics and genomics : MGG · 2019Article
- Induction of Natural Competence in Genetically-modifiedBio-protocol · 2018Article
- Unleashing Natural Competence in Lactococcus lactis by Induction of the Competence Regulator ComX.Applied and environmental microbiology · 2017Article
- Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2.Applied and environmental microbiology · 2017Article
- Regulatory RNAs in the Less Studied Streptococcal Species: From Nomenclature to Identification.Frontiers in microbiology · 2016Review
- Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus thermophilus.Frontiers in microbiology · 2015Article
- Multiplex genome editing by natural transformation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The naturally competent strain Streptococcus thermophilus LMD-9 as a new tool to anchor heterologous proteins on the cell surface.Microbial cell factories · 2014Article
- Peptide pheromone signaling in Streptococcus and Enterococcus.FEMS microbiology reviews · 2014Review
- Bacterial transformation: distribution, shared mechanisms and divergent control.Nature reviews. Microbiology · 2014Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus thermophilus is widely used for the manufacture of yoghurt and Swiss or Italian-type cheeses. These products have a market value of approximately 40 billion dollars per year, making S. thermophilus a species that has major economic importance. Even though the fermentation properties of this bacterium have been gradually improved by classical methods, there is great potential for further improvement through genetic engineering. Due to the recent publication of three complete genome sequences, it is now possible to use a rational approach for designing S. thermophilus starter strains with improved properties. Progress in this field, however, is hampered by a lack of genetic tools. Therefore, we developed a system, based on natural transformation, which makes genetic manipulations in S. thermophilus easy, rapid, and highly efficient. The efficiency of this novel tool should make it possible to construct food-grade mutants of S. thermophilus, opening up exciting new possibilities that should benefit consumers as well as the dairy industry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.