ArticleJournal of clinical microbiology2006
Evaluation of peptide nucleic acid-fluorescence in situ hybridization for identification of clinically relevant mycobacteria in clinical specimens and tissue sections.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- Evaluation of the diagnostic efficiency of fluorescenceFrontiers in medicine · 2024Pooled it
- Use of peptide nucleic acid fluorescent in situ hybridization technique in the detection of Mycobacterium avium paratuberculosis in fecal samples from dairy herds.Veterinary research communications · 2026Article
- Mycobacterium chelonae infection of a cardiovascular bioprosthesis linked to a recent outbreak.Infection · 2025Article
- Biofilm infections of endobronchial valves in COPD patients after endoscopic lung volume reduction: a pilot study with FISHseq.Scientific reports · 2024Article
- Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals.Veterinary sciences · 2024Review
- Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.Journal of fish diseases · 2023Article
- Artificial genetic polymers against human pathologies.Biology direct · 2022Review
- Fluorescence In Situ Hybridization (FISH) Tests for Identifying Protozoan and Bacterial Pathogens in Infectious Diseases.Diagnostics (Basel, Switzerland) · 2022Review
- Biofilm formation in the lung contributes to virulence and drug tolerance of Mycobacterium tuberculosis.Nature communications · 2021Article
- Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Fluorescent Hybridization of Mycobacterium leprae in Skin Samples Collected in Burkina Faso.Journal of clinical microbiology · 2020Article
- Clinical Utility of PNA-FISH for Burn Wound Diagnostics: A Noninvasive, Culture-Independent Technique for Rapid Identification of Pathogenic Organisms in Burn Wounds.Journal of burn care & research : official publication of the American Burn Association · 2019Article
- Leprosy in French Polynesia.New microbes and new infections · 2019Article
- FISHing Mycobacterium tuberculosis Complex by Use of aJournal of clinical microbiology · 2018Article
- FluorescenceJournal of clinical microbiology · 2018Article
- Quantitative assessment of individual populations within polymicrobial biofilms.Scientific reports · 2018Article
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- Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Malaria in Endemic Areas.PloS one · 2015Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With fluorescently labeled PNA (peptide nucleic acid) probes targeting 16S rRNA, we established a 3-h fluorescence in situ hybridization (FISH) procedure for specific visualization of members of the Mycobacterium tuberculosis complex, M. leprae, M. avium, and M. kansasii. Probe specificity was tested against a panel of 25 Mycobacterium spp. and 10 gram-positive organisms. After validation, probes were used to identify 52 mycobacterial culture isolates. Results were compared to conventional genotypic identification with amplification-based methods. All isolates (M. tuberculosis complex, n = 24; M. avium, n = 7; M. kansasii, n = 1) were correctly identified by FISH. In addition, the technique was used successfully for visualization of mycobacteria in biopsies from infected humans or animals. In conclusion, PNA-FISH is a fast and accurate tool for species-specific identification of culture-grown mycobacteria and for direct visualization of these organisms in tissue sections. It may be used successfully for both research and clinical microbiology.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.