ArticleJournal of clinical microbiology2006
Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using theFrontiers in microbiology · 2024Pooled it
- Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review.Microorganisms · 2024Review
- Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation ofReports of biochemistry & molecular biology · 2020Article
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- Molecular Detection of Fastidious and Common Bacteria as Well as Plasmodium spp. in Febrile and Afebrile Children in Franceville, Gabon.The American journal of tropical medicine and hygiene · 2015Article
- Prevalence and drug resistance of nontuberculous mycobacteria, northern China, 2008-2011.Emerging infectious diseases · 2014Article
- A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.PloS one · 2014Article
- Article
- Advantages of using matrix-assisted laser desorption ionization-time of flight mass spectrometry as a rapid diagnostic tool for identification of yeasts and mycobacteria in the clinical microbiological laboratory.Journal of clinical microbiology · 2013Article
- Transmission of Mycobacterium tuberculosis from an Asian elephant (Elephas maximus) to a chimpanzee (Pan troglodytes) and humans in an Australian zoo.Epidemiology and infection · 2013Article
- Reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents.Indian journal of microbiology · 2013Article
- Tuberculosis diagnostics: Challenges and opportunities.Lung India : official organ of Indian Chest Society · 2012Article
- Molecular techniques for pathogen identification and fungus detection in the environment.IMA fungus · 2011Article
- Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections.Clinical microbiology reviews · 2011Review
- Multiplex PCR and reverse line blot hybridization assay (mPCR/RLB).Journal of visualized experiments : JoVE · 2011Article
- Identification of pathogenic Nocardia species by reverse line blot hybridization targeting the 16S rRNA and 16S-23S rRNA gene spacer regions.Journal of clinical microbiology · 2010Article
- Identification and differentiation of clinically relevant mycobacterium species directly from acid-fast bacillus-positive culture broth.Journal of clinical microbiology · 2009Article
- Detection of Enterobacter sakazakii and other pathogens associated with infant formula powder by use of a DNA microarray.Journal of clinical microbiology · 2009Article
- Association between 16S-23S internal transcribed spacer sequence groups of Mycobacterium avium complex and pulmonary disease.Journal of clinical microbiology · 2008Article
- New method for simultaneous species-specific identification of equine strongyles (nematoda, strongylida) by reverse line blot hybridization.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validated by using 78 reference strains belonging to 55 Mycobacterium species, 219 clinical isolates which had been identified as mycobacteria by high-performance liquid chromatography or gas chromatography, three skin biopsy specimens from patients with suspected leprosy which had been shown to contain acid-fast bacilli, and isolates of 14 nonmycobacterial species. All mycobacteria were amplified in the PCR and hybridized with a genus-specific probe (probe MYC). The 34 species-specific probes designed in this study hybridized only with the corresponding Mycobacterium species. The mycobacterial PCR-RLB assay is an efficient tool for the identification of clinical isolates of mycobacteria; it can reliably identify mixed mycobacterial cultures and M. leprae in skin biopsy specimens.
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