ArticleJournal of clinical microbiology2006
Molecular identification of black-grain mycetoma agents.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The trial behind it
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Who cites it
32 citing papers in PubMed, 98 citations in OpenAlex.
- A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species.PloS one · 2014Trial
- A Draft Genome Assembly for the Neglected Tropical Fungal Disease Pathogen Emarellia grisea and Comparison to Other Pleosporalean Agents Causing Eumycetoma in Humans.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- An updated list of eumycetoma causative agents and their differences in grain formation and treatment response.Clinical microbiology reviews · 2024Review
- Changes in fungal taxonomy: mycological rationale and clinical implications.Clinical microbiology reviews · 2023Review
- Epidemiologic Aspects of Mycetoma in Africa.Journal of fungi (Basel, Switzerland) · 2022Review
- Mycetoma: Development of Diagnosis and Treatment.Journal of fungi (Basel, Switzerland) · 2022Review
- Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran.Current medical mycology · 2021Article
- A Short-Tandem-Repeat Assay (Journal of clinical microbiology · 2021Article
- Madurella mycetomatis causing eumycetoma medical treatment: The challenges and prospects.PLoS neglected tropical diseases · 2020Review
- Successful Terbinafine Treatment for Cutaneous Phaeohyphomycosis Caused by Trematosphaeria grisea in a Heart Transplanted Man: Case Report and Literature Review.Mycopathologia · 2020Review
- The genus Madurella: Molecular identification and epidemiology in Sudan.PLoS neglected tropical diseases · 2020Article
- Epidemiological profile and spectrum of neglected tropical disease eumycetoma from Delhi, North India.Epidemiology and infection · 2019Article
- The Accuracy of Histopathological and Cytopathological Techniques in the Identification of the Mycetoma Causative Agents.PLoS neglected tropical diseases · 2019Article
- Mycetoma laboratory diagnosis: Review article.PLoS neglected tropical diseases · 2017Review
- Rapid and Robust Identification of the Agents of Black-Grain Mycetoma by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.Journal of clinical microbiology · 2017Article
- Painless swelling of the forefoot and recurrent subcutaneous abscesses of the lower leg-Two distinct presentations illustrating the spectrum of eumycetoma in a nonendemic country.PLoS neglected tropical diseases · 2017Article
- Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov.Journal of clinical microbiology · 2016Article
- Rapid identification of fungi in culture-negative clinical blood and respiratory samples by DNA sequence analyses.BMC research notes · 2016Article
- Cutaneous phaeohyphomycosis caused by Biatriospora mackinnonii in a renal transplant recipient.JAAD case reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Black-grain mycetomas are subcutaneous devastating chronic infections due to several dematiaceous fungi. They are diagnosed mostly in tropical countries. Identification of these fungi with standard mycological procedures is difficult because of their poor or delayed sporulation. The aim of this study was thus to assess the accuracy of molecular identification of these fungi. A total of 54 strains, mostly of clinical origin, were used, including 15 Madurella mycetomatis, 6 Madurella grisea, 12 Leptosphaeria senegalensis, 4 Leptosphaeria tompkinsii, 6 Pyrenochaeta spp., 4 Curvularia lunata, and 7 Exophiala jeanselmei strains. The internal transcribed spacer 1 (ITS1)-5.8S-ITS2 DNA region was amplified by using universal fungal primers and sequenced. Both intra- and interspecies sequence similarities were assessed. Madurella mycetomatis appeared to be a homogeneous species. More intraspecies variations were found for C. lunata and E. jeanselmei, leading, in some instances, to changes in the initial identification. L. senegalensis and L. tompkinsii showed intraspecies similarities of >99%, but similarity between the two species was <88%. Intergenera and interspecies variations were important, with sequence homologies of <81% between genera. In contrast, Pyrenochaeta romeroi and M. grisea appeared to be heterogeneous, with intraspecies similarities of 40 to 100% and 53 to 100%, respectively, which suggest either erroneous identification or the need for taxonomic revision. Epidemiological and therapeutic studies could benefit from a precise identification of the fungi responsible for black-grain mycetoma based not only on phenotypical characteristics but also on ITS sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.