ArticleJournal of clinical microbiology2006
Development of a serotype-specific DNA microarray for identification of some Shigella and pathogenic Escherichia coli strains.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
- A Health Threat from Farm to Fork: Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024Article
- Molecular serotyping of Shiga toxin-producingVeterinary research forum : an international quarterly journal · 2023Article
- Evidence-Based Tracking of MDRAntibiotics (Basel, Switzerland) · 2021Article
- TCF-ALP: a fluorescent probe for the selective detection ofBiomaterials science · 2021Article
- Genetic Analysis and Serological Detection of Novel O-Antigen Gene Clusters ofJournal of microbiology and biotechnology · 2021Article
- O-Antigen Gene Clusters ofFrontiers in microbiology · 2019Article
- Molecular Tools To Study Preharvest Food Safety Challenges.Microbiology spectrum · 2018Review
- DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases.PloS one · 2015Article
- Novel microarray design for molecular serotyping of shiga toxin- producing Escherichia coli strains isolated from fresh produce.Applied and environmental microbiology · 2014Article
- Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens.PloS one · 2014Article
- Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations.Frontiers in microbiology · 2014Review
- Development of a DNA microarray method for detection and identification of all 15 distinct O-antigen forms of Legionella pneumophila.Applied and environmental microbiology · 2013Article
- A DNA microarray for the versatile diagnosis of infectious diarrhea.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2013Article
- Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products.Applied and environmental microbiology · 2011Article
- Molecular and nanotechnologic approaches to etiologic diagnosis of infectious syndromes.Molecular diagnosis & therapy · 2011Review
- Detection of representative enteropathogenic bacteria, Vibrio spp., pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, using a virulence factor gene-based oligonucleotide microarray.Journal of microbiology (Seoul, Korea) · 2010Article
- Development of a DNA microarray for detection and serotyping of enterotoxigenic Escherichia coli.Journal of clinical microbiology · 2010Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Potentials and limitations of molecular diagnostic methods in food safety.Genes & nutrition · 2009Article
- Food microbial pathogen detection and analysis using DNA microarray technologies.Foodborne pathogens and disease · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella and pathogenic Escherichia coli are major causes of human infectious diseases and are responsible for millions of cases of diarrhea worldwide every year. A convenient and rapid method to identify highly pathogenic serotypes of Shigella and E. coli is needed for large-scale epidemiologic study, timely clinical diagnosis, and reliable quarantine of the pathogens. In this study, a DNA microarray targeting O-serotype-specific genes was developed to detect 15 serotypes of Shigella and E. coli, including Shigella sonnei; Shigella flexneri type 2a; Shigella boydii types 7, 9, 13, 16, and 18; Shigella dysenteriae types 4, 8, and 10; and E. coli O55, O111, O114, O128, and O157. The microarray was tested against 186 representative strains of all Shigella and E. coli O serotypes, 38 clinical isolates, and 9 strains of other bacterial species that are commonly present in stool samples and was shown to be specific and reproducible. The detection sensitivity was 50 ng genomic DNA or 10(4) CFU per ml in mock stool specimens. This is the first report of a microarray for serotyping Shigella and pathogenic E. coli. The method has a number of advantages over traditional bacterial culture and antiserum agglutination methods and is promising for applications in basic microbiological research, clinical diagnosis, food safety, and epidemiological surveillance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.