ArticleThe Histochemical journal1990
Pre-epithelial mucus layer in the colon of conventional and germ-free rats.
Article in The Histochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 109 citations in OpenAlex.
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- Pseudomyxoma Peritonei:Sultan Qaboos University medical journal · 2025Article
- Exploring the role of gut microbiota in colorectal liver metastasis through the gut-liver axis.Frontiers in cell and developmental biology · 2025Review
- Goblet cells: guardians of gut immunity and their role in gastrointestinal diseases.eGastroenterology · 2024Article
- Probiotics and Non-Alcoholic Fatty Liver Disease: Unveiling the Mechanisms ofFoods (Basel, Switzerland) · 2024Review
- Associations of Neonatal Dairy Calf Faecal Microbiota with Inflammatory Markers and Future Performance.Animals : an open access journal from MDPI · 2024Article
- Re-evaluation of dietary interventions in rheumatoid arthritis: can we improve patient conversations around food choices?Rheumatology international · 2024Review
- Repairing gut barrier by traditional Chinese medicine: roles of gut microbiota.Frontiers in cellular and infection microbiology · 2024Review
- Impacts of maternal microbiota and microbial metabolites on fetal intestine, brain, and placenta.BMC biology · 2023Article
- Intra-Amniotic Administration of Cashew Nut (Nutrients · 2023Article
- Utilizing the Gastrointestinal Microbiota to Modulate Cattle Health through the Microbiome-Gut-Organ Axes.Microorganisms · 2022Review
- Fecal Stream Diversion Changes Intestinal Environment, Modulates Mucosal Barrier, and Attenuates Inflammatory Cells in Crohn's Disease.Digestive diseases and sciences · 2022Article
- Vocal fold mucus layer: Comparison of histological protocols for visualization in mice.Laryngoscope investigative otolaryngology · 2022Article
- Impact of antibiotics on the human microbiome and consequences for host health.MicrobiologyOpen · 2022Review
- Integrity of the Intestinal Barrier: The Involvement of Epithelial Cells and Microbiota-A Mutual Relationship.Animals : an open access journal from MDPI · 2022Review
- Liangxue Tongyu Prescription Alleviates Brain Damage in Acute Intracerebral Hemorrhage Rats by Regulating Intestinal Mucosal Barrier Function.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Managing Gut Microbiota through In Ovo Nutrition Influences Early-Life Programming in Broiler Chickens.Animals : an open access journal from MDPI · 2021Review
- The Interplay between Nutrition, Innate Immunity, and the Commensal Microbiota in Adaptive Intestinal Morphogenesis.Nutrients · 2021Review
- Changes in dietary fiber intake in mice reveal associations between colonic mucinGut microbes · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pre-epithelial mucus layer (PML) and epithelial mucins were studied by mucin histochemistry in 10 microns-thick celloidinstabilized cryostat sections in the proximal and distal colon of conventional and germ-free rats aged 120 and 350 days. No continuous PML was found in the proximal colon. A continuous mucus blanket, of fairly homogenous thickness, was observed in the distal colon, where the PML-thickness was 40 +/- 24 microns at 120 days of age and 44 +/- 22 microns at 350 days of age in conventional rats, and 25 +/- 17 microns (120 days) and 22 +/- 10 microns (350 days) in germ-free rats. The stainability of the PML by periodic acid-Schiff and Alcian Blue at pH 2.5 and 1.0 was stronger in conventional rats than in germ-free rats, indicating higher concentrations of mucosubstances and of acid and sulphated mucins, respectively. The PML of the conventional rat distal colon showed a stratified structure of up to eight sublayers. In the distal colon of germ-free rats, the whole gut wall thickness was reduced 47% compared to the conventional rat (germ-free; 185 +/- 73 microns, conventional: 350 +/- 115 microns). No stratification of the PML was observed. The presence of intestinal microflora obviously had a strong influence on the thickness, compactness, mucin content, mucin composition and structure of the pre-epithelial mucus layer.
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