ArticleProtoplasma2006
Possible association of actin filaments with chloroplasts of spinach mesophyll cells in vivo and in vitro.
Article in Protoplasma, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- HSP90.2 promotes COPlant biotechnology journal · 2023Article
- Comparative Proteomic and Physiological Analysis Reveals the Variation Mechanisms of Leaf Coloration and Carbon Fixation in a Xantha Mutant of Ginkgo biloba L.International journal of molecular sciences · 2016Article
- Chloroplast and nuclear photorelocation movements.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
- Evolution of the Cp-Actin-based Motility System of Chloroplasts in Green Plants.Frontiers in plant science · 2016Article
- Distribution pattern changes of actin filaments during chloroplast movement in Adiantum capillus-veneris.Journal of plant research · 2012Article
- Chloroplast actin filaments organize meshwork on the photorelocated chloroplasts in the moss Physcomitrella patens.Planta · 2011Article
- Why have chloroplasts developed a unique motility system?Plant signaling & behavior · 2010Review
- Light, genotype, and abscisic acid affect chloroplast positioning in guard cells of Arabidopsis thaliana leaves in distinct ways.Photosynthesis research · 2010Article
- AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.Molecular & cellular proteomics : MCP · 2010Article
- Is chloroplast import of photosynthesis proteins facilitated by an actin-TOC-TIC-VIPP1 complex?Plant signaling & behavior · 2009Article
- Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Interaction of actin and the chloroplast protein import apparatus.The Journal of biological chemistry · 2009Article
- Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement.Plant physiology · 2008Article
- Article
- Arabidopsis thaliana leaves with altered chloroplast numbers and chloroplast movement exhibit impaired adjustments to both low and high light.Journal of experimental botany · 2008Article
- Membrane contact sites: physical attachment between chloroplasts and endoplasmic reticulum revealed by optical manipulation.Plant signaling & behavior · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In palisade mesophyll cells of spinach (Spinacia oleracea L.) kept under low-intensity white light, chloroplasts were apparently immobile and seemed to be surrounded by fine bundles of actin filaments. High-intensity blue light induced actin-dependent chloroplast movement concomitant with the appearance of a couple of long, straight bundles of actin filaments in each cell, whereas high-intensity red light was essentially ineffective in inducing these responses. The actin organization observed under low-intensity white light has been postulated to function in anchoring chloroplasts at proper intracellular positions through direct interaction with the chloroplasts. Intact chloroplasts, which retained their outer envelopes, were isolated after homogenization of leaves and Percoll centrifugation. No endogenous actin was detected by immunoblotting in the final intact-chloroplast fraction prepared from the leaves kept under low-intensity white light or in darkness. In cosedimentation assays with exogenously added skeletal muscle filamentous actin, however, actin was detected in the intact-chloroplast fraction precipitated after low-speed centrifugation. The association of actin with chloroplasts was apparently dependent on incubation time and chloroplast density. After partial disruption of the outer envelope of isolated chloroplasts by treatment with trypsin, actin was no longer coprecipitated. The results suggest that chloroplasts in spinach leaves can directly interact with actin, and that this interaction may be involved in the regulation of intracellular positioning of chloroplasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.