ArticleJournal of the American Chemical Society2006
Distance-dependent emission from dye-labeled oligonucleotides on striped Au/Ag nanowires: effect of secondary structure and hybridization efficiency.
Article in Journal of the American Chemical Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 72 citations in OpenAlex.
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- Multiplex detection of B-type natriuretic peptide, cardiac troponin I and C-reactive protein with photonic suspension array.PloS one · 2012Article
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- Direct observation of chemokine receptors 5 on T-lymphocyte cell surfaces using fluorescent metal nanoprobes 2: Approximation of CCR5 populations.Biochemical and biophysical research communications · 2011Article
- Fluorescent Metal Nanoshells: Lifetime-Tunable Molecular Probes in Fluorescent Cell Imaging.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011Article
- Hybridization efficiency of molecular beacons bound to gold nanowires: effect of surface coverage and target length.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Nanoscale interfaces to biology.Current opinion in chemical biology · 2010Review
- Understanding the role of thiol and disulfide self-assembled DNA receptor monolayers for biosensing applications.European biophysics journal : EBJ · 2010Article
- Tailoring DNA structure to increase target hybridization kinetics on surfaces.Journal of the American Chemical Society · 2010Article
- Luminescent Silica Core / Silver Shell Encapsulated with Eu(III) Complex.The journal of physical chemistry. C, Nanomaterials and interfaces · 2009Article
- Nano-flares for mRNA regulation and detection.ACS nano · 2009Article
- Viral detection using DNA functionalized gold filaments.The Analyst · 2009Article
- Fluorescent avidin-bound silver particle: a strategy for single target molecule detection on a cell membrane.Analytical chemistry · 2009Article
- Enhanced fluorescence images for labeled cells on silver island films.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- Molecular beacon-metal nanowire interface: effect of probe sequence and surface coverage on sensor performance.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- Plasmon-Coupled Fluorescence Probes: Effect of Emission Wavelength on Fluorophore-Labeled Silver Particles.The journal of physical chemistry. C, Nanomaterials and interfaces · 2008Article
- Single-cell fluorescence imaging using metal plasmon-coupled probe 2: single-molecule counting on lifetime image.Nano letters · 2008Article
- Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays.Journal of the American Chemical Society · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
When fluorescently tagged oligonucleotides are located near metal surfaces, their emission intensity is impacted by both electromagnetic effects (i.e., quenching and/or enhancement of emission) and the structure of the nucleic acids (e.g., random coil, hairpin, or duplex). We present experiments exploring the effect of label position and secondary structure in oligonucleotide probes as a function of hybridization buffer, which impacts the percentage of double-stranded probes on the surface after exposure to complementary DNA. Nanowires containing identifiable patterns of Au and Ag segments were used as the metal substrates in this work, which enabled us to directly compare different dye positions in a single multiplexed experiment and differences in emission for probes attached to the two metals. The observed metal-dye separation dependence for unstructured surface-bound oligonucleotides is highly sensitive to hybridization efficiency, due to substantial changes in DNA extension from the surface upon hybridization. In contrast, fluorophore labeled oligonucleotides designed to form hairpin secondary structures analogous to solution-phase molecular beacon probes are relatively insensitive to hybridization efficiency, since the folded form is quenched and therefore does not appreciably impact the observed distance-dependence of the response. Differences in fluorescence patterning on Au and Ag were noted as a function of not only chromophore identity but also metal-dye separation. For example, emission intensity for TAMRA-labeled oligonucleotides changed from brighter on Ag for 24-base probes to brighter on Au for 48-base probes. We also observed fluorescence enhancement at the ends of nanowires and at surface defects where heightened electromagnetic fields affect the fluorescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.