ArticleJournal of bacteriology2006
Differential regulation of the three methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Pyrrolysine supply and demand shapes the translational landscape inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Methanol-specific methyltransferase isozymes have large carbon kinetic isotope effects that impact methane isotopic signatures.Applied and environmental microbiology · 2026Article
- Methanosarcina acetivorans requires methanol:coenzyme M methyltransferases for ethane formation from ethanol.Antonie van Leeuwenhoek · 2025Article
- A promoter-RBS library for fine-tuning gene expression inApplied and environmental microbiology · 2024Article
- Methylamine-specific methyltransferase paralogs in Methanosarcina are functionally distinct despite frequent gene conversion.The ISME journal · 2019Article
- A Membrane-Bound Cytochrome EnablesmBio · 2019Article
- Growth Characteristics ofArchaea (Vancouver, B.C.) · 2017Article
- Genome-wide gene expression and RNA half-life measurements allow predictions of regulation and metabolic behavior in Methanosarcina acetivorans.BMC genomics · 2016Article
- Genomic and phenotypic differentiation among Methanosarcina mazei populations from Columbia River sediment.The ISME journal · 2015Article
- Genetic basis for metabolism of methylated sulfur compounds in Methanosarcina species.Journal of bacteriology · 2015Article
- Mining proteomic data to expose protein modifications in Methanosarcina mazei strain Gö1.Frontiers in microbiology · 2015Article
- Genetic resources for methane production from biomass described with the Gene Ontology.Frontiers in microbiology · 2014Article
- Towards a computational model of a methane producing archaeum.Archaea (Vancouver, B.C.) · 2014Article
- Carbon source-dependent expansion of the genetic code in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains.Nucleic acids research · 2012Article
- Function and regulation of isoforms of carbon monoxide dehydrogenase/acetyl coenzyme A synthase in Methanosarcina acetivorans.Journal of bacteriology · 2012Article
- Genetic manipulation of Methanosarcina spp.Frontiers in microbiology · 2012Article
- Article
- The appearance of pyrrolysine in tRNAHis guanylyltransferase by neutral evolution.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Physiology and posttranscriptional regulation of methanol:coenzyme M methyltransferase isozymes in Methanosarcina acetivorans C2A.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic analysis of the three methanol-specific methyltransferase 1 operons (mtaCB1, mtaCB2, and mtaCB3) in Methanosarcina acetivorans led to the suggestion that each of them has a discrete function during growth on methanol, which might be reflected in differential gene regulation (Pritchett and Metcalf, Mol. Microbiol. 56:1183-1194, 2005). To test this suggestion, reporter gene fusions were constructed for each of the three operons, and their expression was examined under various growth conditions. Expression of the mtaCB1 and mtaCB2 fusions was 100-fold and 575-fold higher, respectively, in methanol-grown cells than in trimethylamine (TMA)-grown cells. The mtaCB3 fusion was expressed at low levels on methanol, TMA, and dimethylamine but was significantly upregulated on monomethylamine and acetate. When TMA- or acetate-grown cultures were shifted to methanol, the mtaCB1 fusion was expressed most highly during exponential phase, whereas the mtaCB2 fusion, although strongly induced prior to mtaCB1 expression, did not reach full expression levels until stationary phase. The mtaCB3 fusion was transiently expressed prior to entry into exponential phase during a TMA-to-methanol substrate shift experiment. When acetate-grown cells were shifted to medium containing both TMA and methanol, TMA utilization commenced prior to utilization of methanol; however, these two substrates were consumed simultaneously later in growth. Under these conditions expression of the mtaCB2 and mtaCB3 fusions was delayed, suggesting that methylamines may repress their expression.
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