ArticleJournal of bacteriology2006
Regulation of mercury resistance in the crenarchaeote Sulfolobus solfataricus.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- The Emerging Trend of Bio-Engineering Approaches for Microbial Nanomaterial Synthesis and Its Applications.Frontiers in microbiology · 2021Review
- Nonmutational mechanism of inheritance in the ArchaeonProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Metal-tolerant thermophiles: metals as electron donors and acceptors, toxicity, tolerance and industrial applications.Environmental science and pollution research international · 2018Review
- Low-Molecular-Weight Thiols and Thioredoxins Are Important Players in Hg(II) Resistance in Thermus thermophilus HB27.Applied and environmental microbiology · 2018Article
- Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution.Applied and environmental microbiology · 2016Article
- Membrane Association and Catabolite Repression of the Sulfolobus solfataricus α-Amylase.Microorganisms · 2015Article
- Host and viral transcriptional regulators in Sulfolobus: an overview.Extremophiles : life under extreme conditions · 2013Review
- Role of MerH in mercury resistance in the archaeon Sulfolobus solfataricus.Microbiology (Reading, England) · 2013Article
- Mechanisms of metal resistance and homeostasis in haloarchaea.Archaea (Vancouver, B.C.) · 2013Review
- Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula.Journal of bacteriology · 2012Article
- Carbohydrate hydrolysis and transport in the extreme thermoacidophile Sulfolobus solfataricus.Applied and environmental microbiology · 2012Article
- Mercury resistance and mercuric reductase activities and expression among chemotrophic thermophilic Aquificae.Applied and environmental microbiology · 2012Article
- Survival of the fittest: overcoming oxidative stress at the extremes of Acid, heat and metal.Life (Basel, Switzerland) · 2012Review
- The mercury resistance operon: from an origin in a geothermal environment to an efficient detoxification machine.Frontiers in microbiology · 2012Article
- Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius.Frontiers in microbiology · 2012Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
- Acidophilic bacteria and archaea: acid stable biocatalysts and their potential applications.Extremophiles : life under extreme conditions · 2012Review
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Mercuric ion, Hg(II), inactivates generalized transcription in the crenarchaeote Sulfolobus solfataricus. Metal challenge simultaneously derepresses transcription of mercuric reductase (merA) by interacting with the archaeal transcription factor aMerR. Northern blot and primer extension analyses identified two additional Hg(II)-inducible S. solfataricus genes, merH and merI (SSO2690), located on either side of merA. Transcription initiating upstream of merH at promoter merHp was metal inducible and extended through merA and merI, producing a merHAI transcript. Northern analysis of a merRA double mutant produced by linear DNA recombination demonstrated merHp promoter activity was dependent on aMerR to overcome Hg(II) transcriptional inhibition. Unexpectedly, in a merA disruption mutant, the merH transcript was transiently induced after an initial period of Hg(II)-mediated transcription inhibition, indicating continued Hg(II) detoxification. Metal challenge experiments using mutants created by markerless exchange verified the identity of the MerR binding site as an inverted repeat (IR) sequence overlapping the transcription factor B binding recognition element of merHp. The interaction of recombinant aMerR with merHp DNA, studied using electrophoretic mobility shift analysis, demonstrated that complex formation was template specific and dependent on the presence of the IR sequence but insensitive to Hg(II) addition and site-specific IR mutations that relieved in vivo merHp repression. Despite containing a motif resembling a distant ArsR homolog, these results indicate aMerR remains continuously DNA bound to protect and coordinate Hg(II)-responsive control over merHAI transcription. The new genetic methods developed in this work will promote experimental studies on S. solfataricus and other Crenarchaeota.
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