ArticleMolecular and cellular biology2006
Interaction between ROCK II and nucleophosmin/B23 in the regulation of centrosome duplication.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 92 citations in OpenAlex.
- Sensing centrosome amplification: the interface between centriole duplication and autophagy.Nature communications · 2026Article
- RhoA-ROCK2 signaling possesses complex pathophysiological functions in cancer progression and shows promising therapeutic potential.Cancer cell international · 2024Review
- miR-15a targets the HSP90 co-chaperone Morgana in chronic myeloid leukemia.Scientific reports · 2024Article
- Article
- Knockdown of XIST Attenuates Cerebral Ischemia/Reperfusion Injury Through Regulation of miR-362/ROCK2 Axis.Neurochemical research · 2021Article
- Review
- Article
- Rocking the Boat: The Decisive Roles of Rho Kinases During Oocyte, Blastocyst, and Stem Cell Development.Frontiers in cell and developmental biology · 2020Review
- RhoC regulates radioresistance via crosstalk of ROCK2 with the DNA repair machinery in cervical cancer.Journal of experimental & clinical cancer research : CR · 2019Article
- miR‑335 promotes stress granule formation to inhibit apoptosis by targeting ROCK2 in acute ischemic stroke.International journal of molecular medicine · 2019Article
- Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells.Scientific reports · 2019Article
- ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells.Scientific reports · 2018Article
- Rho-Associated Coiled-Coil Kinase (ROCK) in Molecular Regulation of Angiogenesis.Theranostics · 2018Review
- Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.Nature communications · 2017Article
- APC/CProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The ATM- and ATR-related SCD domain is over-represented in proteins involved in nervous system development.Scientific reports · 2016Article
- Cancer Stem Cells and Radioresistance: Rho/ROCK Pathway Plea Attention.Stem cells international · 2016Review
- Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.Oncotarget · 2015Article
- The double face of Morgana in tumorigenesis.Oncotarget · 2015Review
- Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Nucleophosmin (NPM)/B23 has been implicated in the regulation of centrosome duplication. NPM/B23 localizes between two centrioles in the unduplicated centrosome. Upon phosphorylation on Thr(199) by cyclin-dependent kinase 2 (CDK2)/cyclin E, the majority of centrosomal NPM/B23 dissociates from centrosomes, but some NPM/B23 phosphorylated on Thr(199) remains at centrosomes. It has been shown that Thr(199) phosphorylation of NPM/B23 is critical for the physical separation of the paired centrioles, an initial event of the centrosome duplication process. Here, we identified ROCK II kinase, an effector of Rho small GTPase, as a protein that localizes to centrosomes and physically interacts with NPM/B23. Expression of the constitutively active form of ROCK II promotes centrosome duplication, while down-regulation of ROCK II expression results in the suppression of centrosome duplication, especially delaying the initiation of centrosome duplication during the cell cycle. Moreover, ROCK II regulates centrosome duplication in its kinase and centrosome localization activity-dependent manner. We further found that ROCK II kinase activity is significantly enhanced by binding to NPM/B23 and that NPM/B23 acquires a higher binding affinity to ROCK II upon phosphorylation on Thr(199). Moreover, physical interaction between ROCK II and NPM/B23 in vivo occurs in association with CDK2/cyclin E activation and the emergence of Thr(199)-phosphorylated NPM/B23. All these findings point to ROCK II as the effector of the CDK2/cyclin E-NPM/B23 pathway in the regulation of centrosome duplication.
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