ArticleBMC microbiology2006
Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed.
- On the role of cell chaining in the attenuation of aVirulence · 2026Article
- Three metabolic pathways replenishing the one-carbon pool collectively support growth and virulence ofeLife · 2026Article
- Bacterial aggregation facilitates internalin-mediated invasion ofFrontiers in cellular and infection microbiology · 2024Article
- Vitamin A Promotes the Fusion of Autophagolysosomes and Prevents Excessive Inflammasome Activation in Dextran Sulfate Sodium-Induced Colitis.International journal of molecular sciences · 2023Article
- Live-cell imaging reveals single-cell and population-level infection strategies ofFrontiers in immunology · 2023Article
- Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms.Nature communications · 2022Article
- Measurement of Protein Mobility inFrontiers in microbiology · 2021Article
- Correlative light and scanning electron microscopy (CLSEM) for analysis of bacterial infection of polarized epithelial cells.Scientific reports · 2019Article
- Food Microstructure and Fat Content Affect Growth Morphology, Growth Kinetics, and Preferred Phase for Cell Growth ofApplied and environmental microbiology · 2019Article
- Stable genetic integration of a red fluorescent protein in a virulent Group AAccess microbiology · 2019Article
- Contribution of toxic shock syndrome toxin-1 to systemic inflammation investigated by a mouse model of cervicovaginal infection with Staphylococcus aureus.Medical microbiology and immunology · 2018Article
- Passive immunization with anti-ActA and anti-listeriolysin O antibodies protects against Listeria monocytogenes infection in mice.Scientific reports · 2016Article
- Trypanosoma cruzi Differentiates and Multiplies within Chimeric Parasitophorous Vacuoles in Macrophages Coinfected with Leishmania amazonensis.Infection and immunity · 2016Article
- Listeria monocytogenes σH Contributes to Expression of Competence Genes and Intracellular Growth.Journal of bacteriology · 2016Article
- Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes.Infection and immunity · 2015Article
- Exploring the diversity of Listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of the honeycomb-like morphotype.Applied and environmental microbiology · 2015Article
- Actin network disassembly powers dissemination of Listeria monocytogenes.Journal of cell science · 2014Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExisting virulence models are often difficult to apply for quantitative comparison of invasion potentials of Listeria monocytogenes. Well-to-well variation between cell-line based in vitro assays is practically unavoidable, and variation between individual animals is the cause of large deviations in the observed capacity for infection when animal models are used. One way to circumvent this problem is to carry out virulence studies as competition assays between 2 or more strains. This, however, requires invasion-neutral markers that enable easy discrimination between the different strains.
resultsA fluorescent marker system, allowing visualization and identification of single L. monocytogenes cells as well as colonies in a non-destructive manner, was developed. Five different fluorescent labels are available, and allowed simultaneous visual discrimination between three differently labelled strains at the single cell level by use of fluorescence microscopy. More than 90% of the L. monocytogenes host cells maintained the fluorescence tags for 40 generations. The fluorescence tags did not alter the invasive capacity of the L. monocytogenes cells in a traditional Caco-2 cell invasion assay, and visual discrimination between invaded bacteria carrying different fluorescent labels inside the cells was possible.
conclusionThe constructed fluorescent marker system is stable, easy to use, does not affect the virulence of L. monocytogenes in Caco-2 cell assays, and allows discrimination between differently labelled bacteria after internalization in these cells.
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