ArticleBiotechnology and bioengineering2007
Metabolic engineering of sesquiterpene metabolism in yeast.
Article in Biotechnology and bioengineering, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 134 citations in OpenAlex.
- Plant sesquiterpene lactones.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
- Article
- Studies on Streptomyces sp. SN-593: reveromycin biosynthesis, β-carboline biomediator activating LuxR family regulator, and construction of terpenoid biosynthetic platform.The Journal of antibiotics · 2022Review
- Article
- Germacrene A-A Central Intermediate in Sesquiterpene Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Combinatorial Metabolic Engineering inBioengineering (Basel, Switzerland) · 2020Article
- Hyper-production of taxol fromBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- High production of valencene in Saccharomyces cerevisiae through metabolic engineering.Microbial cell factories · 2019Article
- Mouse lipogenic proteins promote the co-accumulation of triacylglycerols and sesquiterpenes in plant cells.Planta · 2019Article
- Identifying and engineering the ideal microbial terpenoid production host.Applied microbiology and biotechnology · 2019Review
- Co-production of farnesol and coenzyme QMicrobial cell factories · 2019Article
- Isoprenoid-Based Biofuels: Homologous Expression and Heterologous Expression in Prokaryotes.Applied and environmental microbiology · 2016Review
- Engineering Triterpene and Methylated Triterpene Production in Plants Provides Biochemical and Physiological Insights into Terpene Metabolism.Plant physiology · 2016Article
- Microbial production strategies and applications of lycopene and other terpenoids.World journal of microbiology & biotechnology · 2016Article
- Article
- Stable heterologous expression of biologically active terpenoids in green plant cells.Frontiers in plant science · 2015Review
- Co-opted oxysterol-binding ORP and VAP proteins channel sterols to RNA virus replication sites via membrane contact sites.PLoS pathogens · 2014Article
- Identification of novel knockout targets for improving terpenoids biosynthesis in Saccharomyces cerevisiae.PloS one · 2014Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Terpenes are structurally diverse compounds that are of interest because of their biological activities and industrial value. These compounds consist of chirally rich hydrocarbon backbones derived from terpene synthases, which are subsequently decorated with hydroxyl substituents catalyzed by terpene hydroxylases. Availability of these compounds is, however, limited by intractable synthetic means and because they are produced in low amounts and as complex mixtures by natural sources. We engineered yeast for sesquiterpene accumulation by introducing genetic modifications that enable the yeast to accumulate high levels of the key intermediate farnesyl diphosphate (FPP). Co-expression of terpene synthase genes diverted the enlarged FPP pool to greater than 80 mg/L of sesquiterpene. Efficient coupling of terpene production with hydroxylation was also demonstrated by coordinate expression of terpene hydroxylase activity, yielding 50 mg/L each of hydrocarbon and hydroxylated products. These yeast now provide a convenient format for investigating catalytic coupling between terpene synthases and hydroxylases, as well as a platform for the industrial production of high value, single-entity and stereochemically unique terpenes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.