ArticleActa crystallographica. Section F, Structural biology and crystallization communications2006
A serendipitous discovery that in situ proteolysis is essential for the crystallization of yeast CPSF-100 (Ydh1p).
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 22 citations in OpenAlex.
- Molecular details of the CPSF73-CPSF100 C-terminal heterodimer and interaction with Symplekin.Open biology · 2023Article
- Molecular mechanism for the interaction between human CPSF30 and hFip1.Genes & development · 2020Article
- Recent molecular insights into canonical pre-mRNA 3'-end processing.Transcription · 2020Review
- Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.Molecular cell · 2020Article
- In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices.International journal of molecular sciences · 2020Article
- In Situ Proteolysis for Crystallization of Membrane Bound Cytochrome P450 17A1 and 17A2 Proteins from Zebrafish.Current protocols in protein science · 2016Article
- Protein stability: a crystallographer's perspective.Acta crystallographica. Section F, Structural biology communications · 2016Review
- Cog5-Cog7 crystal structure reveals interactions essential for the function of a multisubunit tethering complex.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Crystal structure of the Rna14-Rna15 complex.RNA (New York, N.Y.) · 2012Article
- Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
- Mass spectrometry guided in situ proteolysis to obtain crystals for X-ray structure determination.Journal of the American Society for Mass Spectrometry · 2010Article
- In situ proteolysis for protein crystallization and structure determination.Nature methods · 2007Article
- The use of in situ proteolysis in the crystallization of murine CstF-77.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
The cleavage and polyadenylation specificity factor (CPSF) complex is required for the cleavage and polyadenylation of the 3'-end of messenger RNA precursors in eukaryotes. During structural studies of the 100 kDa subunit (CPSF-100, Ydh1p) of the yeast CPSF complex, it was serendipitously discovered that a solution that is infected by a fungus (subsequently identified as Penicillium) is crucial for the crystallization of this protein. Further analyses suggest that the protein has undergone partial proteolysis during crystallization, resulting in the deletion of an internal segment of about 200 highly charged and hydrophilic residues, very likely catalyzed by a protease secreted by the fungus. With the removal of this segment, yeast CPSF-100 (Ydh1p) has greatly reduced solubility and can be crystallized in the presence of a minute amount of precipitant.
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