ArticlePlant physiology2006
Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed, 301 citations in OpenAlex.
- SaNAR2.2, A Novel Member of the NAR2 Family from the EuhalophyteMembranes · 2026Article
- Genetically Encoded Fluorescent Biosensors Enable Noninvasive Real-Time Visualization of Nitrate Dynamics in Intact Living Plants.Biosensors · 2026Article
- Article
- Hyperspectral imaging of grains uncovers the genetic architecture of nitrogen response of development in bread wheat.Molecular breeding : new strategies in plant improvement · 2025Article
- Nitrogen-Driven Orchestration of Lateral Root Development: Molecular Mechanisms and Systemic Integration.Biology · 2025Review
- 9311 allele of OsNAR2.2 enhances nitrate transport to improve rice yield and nitrogen use efficiency.Plant biotechnology journal · 2025Article
- BdNRT2A and BdNRT3.2 Are the Major Components of the High-Affinity Nitrate Transport System inPlant direct · 2025Article
- Beyond nitrate transport: AtNRT2.4 responds to local and systemic nitrogen signaling in Arabidopsis.BMC plant biology · 2025Article
- Physio-biochemical and molecular mechanisms of low nitrogen stress tolerance in peanut (Arachis hypogaea L.).Plant molecular biology · 2025Article
- Physiological, molecular, and environmental insights into plant nitrogen uptake, and metabolism under abiotic stresses.The plant genome · 2024Review
- Novel Proteins of the High-Affinity Nitrate Transporter Family NRT2, SaNRT2.1 and SaNRT2.5, from the EuhalophyteInternational journal of molecular sciences · 2024Article
- Overexpression of OsNAR2.1 by OsNAR2.1 promoter increases drought resistance by increasing the expression of OsPLDα1 in rice.BMC plant biology · 2024Article
- NRT1.1B mediates rice plant growth and soil microbial diversity under different nitrogen conditions.AMB Express · 2024Article
- Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis.Journal of experimental botany · 2024Article
- Functional analyses of the NRT2 family of nitrate transporters inFrontiers in plant science · 2024Review
- The Functional Diversity of the High-Affinity Nitrate Transporter Gene Family in Hexaploid Wheat: Insights from Distinct Expression Profiles.International journal of molecular sciences · 2023Article
- Transcriptome Profiling Provides Insights into the Early Development of Tiller Buds in High- and Low-Tillering Orchardgrass Genotypes.International journal of molecular sciences · 2023Article
- Nuclear translocation of OsMADS25 facilitated by OsNAR2.1 in reponse to nitrate signals promotes rice root growth by targeting OsMADS27 and OsARF7.Plant communications · 2023Article
- Identification of genes associated with abiotic stress tolerance in sweetpotato using weighted gene co-expression network analysis.Plant direct · 2023Article
- Nitrogen Journey in Plants: From Uptake to Metabolism, Stress Response, and Microbe Interaction.Biomolecules · 2023Review
51 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The identification of a family of NAR2-type genes in higher plants showed that there was a homolog in Arabidopsis (Arabidopsis thaliana), AtNAR2.1. These genes encode part of a two-component nitrate high-affinity transport system (HATS). As the Arabidopsis NRT2 gene family of nitrate transporters has been characterized, we tested the idea that AtNAR2.1 and AtNRT2.1 are partners in a two-component HATS. Results using the yeast split-ubiquitin system and Xenopus oocyte expression showed that the two proteins interacted to give a functional HATS. The growth and nitrogen (N) physiology of two Arabidopsis gene knockout mutants, atnrt2.1-1 and atnar2.1-1, one for each partner protein, were compared. Both types of plants had lost HATS activity at 0.2 mm nitrate, but the effect was more severe in atnar2.1-1 plants. The relationship between plant N status and nitrate transporter expression revealed a pattern that was characteristic of N deficiency that was again stronger in atnar2.1-1. Plants resulting from a cross between both mutants (atnrt2.1-1 x atnar2.1-1) showed a phenotype like that of the atnar2.1-1 mutant when grown in 0.5 mm nitrate. Lateral root assays also revealed growth differences between the two mutants, confirming that atnar2.1-1 had a stronger phenotype. To show that the impaired HATS did not result from the decreased expression of AtNRT2.1, we tested if constitutive root expression of a tobacco (Nicotiana plumbaginifolia) gene, NpNRT2.1, previously been shown to complement atnrt2.1-1, can restore HATS to the atnar2.1-1 mutant. These plants did not recover wild-type nitrate HATS. Taken together, these results show that AtNAR2.1 is essential for HATS of nitrate in Arabidopsis.
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