ArticleJournal of bacteriology2007
Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature lysis of phage-infected Lactococcus lactis.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 104 citations in OpenAlex.
- Article
- Diverse Bacterial Anti-Phage Strategies: From the Laboratory to the Clinic.Current issues in molecular biology · 2026Review
- Revisiting lactococcal abortive infection-like antiphage systems: diversity, structures, and mechanisms.FEMS microbiology reviews · 2026Review
- In silico approaches for discovering microbial antiviral defense systems.Briefings in bioinformatics · 2025Review
- In silico characterization of defense system hotspots in Acinetobacter spp.Communications biology · 2025Article
- Tail assembly interference is a common strategy in bacterial antiviral defenses.Nature communications · 2024Article
- Bacterial defense systems exhibit synergistic anti-phage activity.Cell host & microbe · 2024Article
- Review
- Anti-phage defence through inhibition of virion assembly.Nature communications · 2024Article
- Identification of HDV-like theta ribozymes involved in tRNA-based recoding of gut bacteriophages.Nature communications · 2024Article
- Infant gut DNA bacteriophage strain persistence during the first 3 years of life.Cell host & microbe · 2024Article
- Article
- The highly diverse antiphage defence systems of bacteria.Nature reviews. Microbiology · 2023Review
- Isolation, Characterization, and Comparative Genomic Analysis of Bacteriophage Ec_MI-02 from Pigeon Feces InfectingInternational journal of molecular sciences · 2023Article
- The Battle between Bacteria and Bacteriophages: A Conundrum to Their Immune System.Antibiotics (Basel, Switzerland) · 2023Review
- Cell wall modifications that alter the exolytic activity of lactococcal phage endolysins have little impact on phage growth.Frontiers in microbiology · 2023Article
- Widespread stop-codon recoding in bacteriophages may regulate translation of lytic genes.Nature microbiology · 2022Article
- Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.FEMS microbiology reviews · 2021Review
- Prophages encode phage-defense systems with cognate self-immunity.Cell host & microbe · 2021Article
- Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems.Nucleic acids research · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA system inhibits both prolate and small isometric phages by interfering with the early stages of phage DNA replication. However, abiA alone does not account for the full abortive activity reported for pTR2030. In this study, a 7.5-kb region positioned within the IS elements and downstream of abiA was sequenced to reveal seven additional open reading frames (ORFs). A single ORF, designated abiZ, was found to be responsible for a significant reduction in plaque size and an efficiency of plaquing (EOP) of 10(-6), without affecting phage adsorption. AbiZ causes phage phi31-infected Lactococcus lactis NCK203 to lyse 15 min early, reducing the burst size of phi31 100-fold. Thirteen of 14 phages of the P335 group were sensitive to AbiZ, through reduction in either plaque size, EOP, or both. The predicted AbiZ protein contains two predicted transmembrane helices but shows no significant DNA homologies. When the phage phi31 lysin and holin genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and lysin caused partial lysis of NCK203. In the presence of AbiZ, lysis occurred 30 min earlier. In holin-induced cells, membrane permeability as measured using propidium iodide was greater in the presence of AbiZ. These results suggest that AbiZ may interact cooperatively with holin to cause premature lysis.
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