ArticleJournal of bacteriology2006
SarA is a repressor of hla (alpha-hemolysin) transcription in Staphylococcus aureus: its apparent role as an activator of hla in the prototype strain NCTC 8325 depends on reduced expression of sarS.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 55 citations in OpenAlex.
- Pathogen Partnerships or Power Struggles? Pseudomonas aeruginosa, and Staphylococcus aureus Dynamics in Cystic Fibrosis.Current microbiology · 2025Article
- A genetic regulatory see-saw of biofilm and virulence in MRSA pathogenesis.Frontiers in microbiology · 2023Review
- Article
- Intracellular Environment andFrontiers in microbiology · 2020Article
- Contribution ofInfection and immunity · 2019Article
- Review
- Insights into the regulation of small RNA expression: SarA represses the expression of two sRNAs in Staphylococcus aureus.Nucleic acids research · 2016Article
- The Effect ofJundishapur journal of microbiology · 2016Article
- RpiRc Is a Pleiotropic Effector of Virulence Determinant Synthesis and Attenuates Pathogenicity in Staphylococcus aureus.Infection and immunity · 2016Article
- Impact of the Regulators SigB, Rot, SarA and sarS on the Toxic Shock Tst Promoter and TSST-1 Expression in Staphylococcus aureus.PloS one · 2015Article
- The catabolite control protein E (CcpE) affects virulence determinant production and pathogenesis of Staphylococcus aureus.The Journal of biological chemistry · 2014Article
- Contribution of teg49 small RNA in the 5' upstream transcriptional region of sarA to virulence in Staphylococcus aureus.Infection and immunity · 2014Article
- Impact of the functional status of saeRS on in vivo phenotypes of Staphylococcus aureus sarA mutants.Molecular microbiology · 2014Article
- Genetic variation in the Staphylococcus aureus 8325 strain lineage revealed by whole-genome sequencing.PloS one · 2013Article
- Emerging functions for the Staphylococcus aureus RNome.PLoS pathogens · 2013Review
- sarA-mediated repression of protease production plays a key role in the pathogenesis of Staphylococcus aureus USA300 isolates.Molecular microbiology · 2012Article
- Genetic regulation of the intercellular adhesion locus in staphylococci.Frontiers in cellular and infection microbiology · 2012Review
- Dual-function RNA regulators in bacteria.Biochimie · 2011Review
- Defining the strain-dependent impact of the Staphylococcal accessory regulator (sarA) on the alpha-toxin phenotype of Staphylococcus aureus.Journal of bacteriology · 2011Article
- Identification of single nucleotide polymorphisms associated with hyperproduction of alpha-toxin in Staphylococcus aureus.PloS one · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most Staphylococcus aureus strains, inactivation of sarA increases hla transcription, indicating that sarA is a repressor. However, in S. aureus NCTC 8325 and its derivatives, used for most studies of hla regulation, inactivation of sarA resulted in decreased hla transcription. The disparate phenotype of strain NCTC 8325 seems to be associated with its rsbU mutation, which leads to sigma(B) deficiency. This has now been verified by the demonstration that sarA repressed hla transcription in an rsbU+ derivative of strain 8325-4 (SH1000). That sarA could act as a repressor of hla in an 8325-4 background was confirmed by the observation that inactivation of sarA in an agr sarS rot triple mutant dramatically increased hla transcription to wild-type levels. However, the apparent role of sarA as an activator of hla in 8325-4 was not a result of the rsbU mutation alone, as inactivation of sarA in another rsbU mutant, strain V8, led to increased hla transcription. Northern blot analysis revealed much higher levels of sarS mRNA in strain V8 than in 8325-4, which was likely due to the mutation in the sarS activator, tcaR, in 8325-4, which was not found in strain V8. On the other hand, the relative increase in sarS transcription upon the inactivation of sarA was 15-fold higher in 8325-4 than in strain V8. Because of this, inactivation of sarA in 8325-4 means a net increase in repressor activity, whereas in strain V8, inactivation of sarA means a net decrease in repressor activity and, therefore, enhanced hla transcription.
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