ArticleJournal of bacteriology2006
Escherichia coli RNA polymerase recognition of a sigma70-dependent promoter requiring a -35 DNA element and an extended -10 TGn motif.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Identification of a novel alternate promoter element in the pheST operon of Escherichia coli.Molecular biology reports · 2024Article
- Diversity of σJournal of bacteriology · 2023Article
- Development of a Metabolite Sensor for High-Throughput Detection of Aldehydes inFrontiers in bioengineering and biotechnology · 2018Article
- PromoterPredict: sequence-based modelling ofPeerJ · 2018Article
- Characterization of a Minimal Type of Promoter Containing the -10 Element and a Guanine at the -14 or -13 Position in Mycobacteria.Journal of bacteriology · 2017Article
- Mix-and-matching as a promoter recognition mechanism by ECF σ factors.BMC evolutionary biology · 2017Article
- Promoter Recognition by Extracytoplasmic Function σ Factors: Analyzing DNA and Protein Interaction Motifs.Journal of bacteriology · 2016Article
- Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis.Scientific reports · 2016Article
- Mutations That Stimulate flhDC Expression in Escherichia coli K-12.Journal of bacteriology · 2015Article
- The STM4195 gene product (PanS) transports coenzyme A precursors in Salmonella enterica.Journal of bacteriology · 2015Article
- Analysis of the Salmonella regulatory network suggests involvement of SsrB and H-NS in σ(E)-regulated SPI-2 gene expression.Frontiers in microbiology · 2015Article
- Pr is a member of a restricted class of σ70-dependent promoters that lack a recognizable -10 element.Nucleic acids research · 2012Article
- Role of ISKpn7 and deletions in blaKPC gene expression.Antimicrobial agents and chemotherapy · 2012Article
- Bacteriophage T4 MotA activator and the β-flap tip of RNA polymerase target the same set of σ70 carboxyl-terminal residues.The Journal of biological chemistry · 2011Article
- Redefining Escherichia coli σ(70) promoter elements: -15 motif as a complement of the -10 motif.Journal of bacteriology · 2011Article
- Genome-wide identification of transcriptional start sites in the plant pathogen Pseudomonas syringae pv. tomato str. DC3000.PloS one · 2011Article
- Reduced capacity of alternative sigmas to melt promoters ensures stringent promoter recognition.Genes & development · 2009Article
- Promoter analysis of macrophage- and tick cell-specific differentially expressed Ehrlichia chaffeensis p28-Omp genes.BMC microbiology · 2009Article
- The promoter spacer influences transcription initiation via sigma70 region 1.1 of Escherichia coli RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Advances in bacterial promoter recognition and its control by factors that do not bind DNA.Nature reviews. Microbiology · 2008Review
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3 authors.
Funding
Abstract
Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report here an investigation of a promoter, P(minor), that has a nearly perfect match to the -35 sequence and has the TGn motif. However, P(minor) contains an extremely poor sigma70 -10 element. We demonstrate that P(minor) is active both in vivo and in vitro and that mutations in either the -35 or the TGn motif eliminate its activity. Mutation of the TGn motif can be compensated for by mutations that make the -10 element more canonical, thus converting the -35/TGn promoter to a -35/-10 promoter. Potassium permanganate footprinting on the nontemplate and template strands indicates that when polymerase is in a stable (open) complex with P(minor), the DNA is single stranded from positions -11 to +4. We also demonstrate that transcription from P(minor) incorporates nontemplated ribonucleoside triphosphates at the 5' end of the P(minor) transcript, which results in an anomalous assignment for the start site when primer extension analysis is used. P(minor) represents one of the few -35/TGn promoters that have been characterized and serves as a model for investigating functional differences between these promoters and the better-characterized -10/-35 and extended -10 promoters used by E. coli RNA polymerase.
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