ArticleNucleic acids research2006
A novel endonuclease IV post-PCR genotyping system.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 9 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 9 syntheses or guidelines pooled it, 85 citations in OpenAlex.
- Genetic predisposition to hypertension is associated with preeclampsia in European and Central Asian women.Nature communications · 2020Pooled it
- Genome-wide common and rare variant analysis provides novel insights into clozapine-associated neutropenia.Molecular psychiatry · 2017Pooled it
- HLA class II sequence variants influence tuberculosis risk in populations of European ancestry.Nature genetics · 2016Pooled it
- Sequence variants in the PTCH1 gene associate with spine bone mineral density and osteoporotic fractures.Nature communications · 2016Pooled it
- Meta-GWAS and Meta-Analysis of Exome Array Studies Do Not Reveal Genetic Determinants of Serum Hepcidin.PloS one · 2016Pooled it
- A meta-analysis of genome-wide association studies identifies novel variants associated with osteoarthritis of the hip.Annals of the rheumatic diseases · 2014Pooled it
- Sequence variants at CYP1A1-CYP1A2 and AHR associate with coffee consumption.Human molecular genetics · 2011Pooled it
- Meta-analysis of genome-wide association studies confirms a susceptibility locus for knee osteoarthritis on chromosome 7q22.Annals of the rheumatic diseases · 2011Pooled it
- Twenty bone-mineral-density loci identified by large-scale meta-analysis of genome-wide association studies.Nature genetics · 2009Pooled it
- Association of BRCA2 K3326* With Small Cell Lung Cancer and Squamous Cell Cancer of the Skin.Journal of the National Cancer Institute · 2018Article
- Endonuclease IV recognizes single base mismatch on the eighth base 3' to the abasic site in DNA strands for ultra-selective and sensitive mutant-type DNA detection.RSC advances · 2018Article
- Sequence variants in ARHGAP15, COLQ and FAM155A associate with diverticular disease and diverticulitis.Nature communications · 2017Article
- Comprehensive population-wide analysis of Lynch syndrome in Iceland reveals founder mutations in MSH6 and PMS2.Nature communications · 2017Article
- Whole-genome sequencing identifies rare genotypes in COMP and CHADL associated with high risk of hip osteoarthritis.Nature genetics · 2017Article
- A rare IL33 loss-of-function mutation reduces blood eosinophil counts and protects from asthma.PLoS genetics · 2017Article
- A genome-wide association study yields five novel thyroid cancer risk loci.Nature communications · 2017Article
- Papillary Thyroid Carcinoma: Association Between Germline DNA Variant Markers and Clinical Parameters.Thyroid : official journal of the American Thyroid Association · 2016Article
- Common variants upstream of KDR encoding VEGFR2 and in TTC39B associate with endometriosis.Nature communications · 2016Article
- Variants with large effects on blood lipids and the role of cholesterol and triglycerides in coronary disease.Nature genetics · 2016Article
- Replication study of 34 common SNPs associated with prostate cancer in the Romanian population.Journal of cellular and molecular medicine · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA. The three component substrate is characterized by single-stranded DNA target, an oligonucleotide probe, separated from a helper oligonucleotide by a one base gap. The oligonucleotide probe contains a non-fluorescent quencher at the 5' end and fluorophore attached to the 3' end through a special rigid linker. Fluorescence of the oligonucleotide probe is efficiently quenched by the interaction of terminal dye and quencher when not hybridized. Upon hybridization of the oligonucleotide probe and helper probe to their complementary target, the phosphodiester linkage between the rigid linker and the 3' end of the probe is efficiently cleaved, generating a fluorescent signal. In this study, the use of the Endo IV assay as a post-PCR amplification detection system is demonstrated. High sensitivity and specificity are illustrated using single nucleotide polymorphism detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.