ArticlePflugers Archiv : European journal of physiology1990
How do patch clamp seals form? A lipid bleb model.
Article in Pflugers Archiv : European journal of physiology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Loose-patch clamp analysis applied to voltage-gated ionic currents following pharmacological ryanodine receptor modulation in murine hippocampal cornu ammonis-1 pyramidal neurons.Frontiers in physiology · 2024Article
- Zingerone Modulates Neuronal Voltage-Gated NaInternational journal of molecular sciences · 2022Article
- The Integrated Effects of Brivaracetam, a Selective Analog of Levetiracetam, on Ionic Currents and Neuronal Excitability.Biomedicines · 2021Article
- Effectiveness of Columbianadin, a Bioactive Coumarin Derivative, in Perturbing Transient and PersistentInternational journal of molecular sciences · 2021Article
- High Capability of Pentagalloylglucose (PGG) in Inhibiting Multiple Types of Membrane Ionic Currents.International journal of molecular sciences · 2020Article
- Cleaning patch-clamp pipettes for immediate reuse.Scientific reports · 2016Article
- Biophysics and structure of the patch and the gigaseal.Biophysical journal · 2009Article
- Voltage-gated proton channels: what's next?The Journal of physiology · 2008Review
- Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording.Biophysical journal · 2007Article
- Gadolinium effects on gigaseal formation and the adhesive properties of a fungal amoeboid cell, the slime mutant of Neurospora crassa.The Journal of membrane biology · 2004Article
- Cl- flux through a non-selective, stretch-sensitive conductance influences the outer hair cell motor of the guinea-pig.The Journal of physiology · 2003Article
- On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes.The Journal of physiology · 2000Article
- Effects of intra- and extracellular acidifications on single channel Kir2.3 currents.The Journal of physiology · 1999Article
- A novel voltage clamp technique for mapping ionic currents from cultured skeletal myotubes.Biophysical journal · 1998Article
- Similarity of ATP-dependent K+ channels in skeletal muscle fibres from normal and mutant mdx mice.The Journal of physiology · 1997Article
- Ion permeation through light-activated channels in rhabdomeric photoreceptors. Role of divalent cations.The Journal of general physiology · 1996Article
- Amiloride-sensitive sodium channels in confluent M-1 mouse cortical collecting duct cells.The Journal of membrane biology · 1995Article
- Hypertonicity activates nonselective cation channels in mouse cortical collecting duct cells.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- A look at membrane patches with a scanning force microscope.Biophysical journal · 1995Article
- Cl- and K+ channel currents during the action potential in Chara. Simultaneous recording of membrane voltage and patch currents.The Journal of membrane biology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Individual ion channels are electrically isolated and studied in living cells with the tight patch voltage clamp method. Channels are identified, categorized, and sometimes named on the basis of the biophysical properties obtained with this method. Although it is usually presumed that these recordings are from native, undisturbed membrane, the physical basis of this technique is not well established. Observations that lipid blebs readily form when suction is applied to patch clamp electrodes suggest that many single channel recordings are from ion channels in these blebs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.