ArticleFEBS letters2006
A single amino acid mutation attenuates rundown of voltage-gated calcium channels.
Article in FEBS letters, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- MARCKS mediates vascular contractility through regulating interactions between voltage-gated CaVascular pharmacology · 2020Article
- Protein phosphorylation maintains the normal function of cloned human CaThe Journal of general physiology · 2018Article
- Effects of salvianolic acid B on L-type calcium channels and myocardial contractility in isolated rat ventricular myocytes and hERG KNaunyn-Schmiedeberg's archives of pharmacology · 2017Article
- Bimodal regulation of an Elk subfamily K+ channel by phosphatidylinositol 4,5-bisphosphate.The Journal of general physiology · 2015Article
- A Polybasic Plasma Membrane Binding Motif in the I-II Linker Stabilizes Voltage-gated CaV1.2 Calcium Channel Function.The Journal of biological chemistry · 2015Article
- A single-cross, RNA interference-based genetic tool for examining the long-term maintenance of homeostatic plasticity.Frontiers in cellular neuroscience · 2015Article
- Voltage-gated Ca2+ channels in accessory lobe neurons of the chick.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2014Article
- Age-related homeostatic midchannel proteolysis of neuronal L-type voltage-gated Ca²⁺ channels.Neuron · 2014Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Channelopathies linked to plasma membrane phosphoinositides.Pflugers Archiv : European journal of physiology · 2010Review
- Regulation of voltage-gated Ca2+ channels by lipids.Cell calcium · 2009Review
- Origin of the voltage dependence of G-protein regulation of P/Q-type Ca2+ channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Atypical properties of a conventional calcium channel beta subunit from the platyhelminth Schistosoma mansoni.BMC physiology · 2008Article
- PIP2 is a necessary cofactor for ion channel function: how and why?Annual review of biophysics · 2008Review
- The lipid connection-regulation of voltage-gated Ca(2+) channels by phosphoinositides.Pflugers Archiv : European journal of physiology · 2007Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The activity of voltage-gated calcium channels (VGCCs) decreases with time in whole-cell and inside-out patch-clamp recordings. In this study we found that substituting a single amino acid (I1520) at the intracellular end of IIIS6 in the alpha(1) subunit of P/Q-type Ca(2+) channels with histidine or aspartate greatly attenuated channel rundown in inside-out patch-clamp recordings. The homologous mutations also slowed rundown of N- and L-type Ca(2+) channels, albeit to a lesser degree. In P/Q-type channels, the attenuation of rundown is accompanied by an increased apparent affinity for phosphatidylinositol-4,5-bisphosphate, which has been shown to be critical for maintaining Ca(2+) channel activity [L. Wu, C.S. Bauer, X.-G. Zhen, C. Xie, J. Yang, Dual regulation of voltage-gated calcium channels by PtdIns(4,5)P2. Nature 419 (2002) 947-952]. Furthermore, the histidine mutation significantly stabilized the open state, making the channels easier to open, slower to close, harder to inactivate and faster to recover from inactivation. Our finding that mutation of a single amino acid can greatly attenuate rundown provides an easy and efficient way to slow the rundown of VGCCs, facilitating functional studies that require direct access to the cytoplasmic side of the channel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.