ArticleMolecular and cellular biology1990
A uridylate tract mediates efficient heterogeneous nuclear ribonucleoprotein C protein-RNA cross-linking and functionally substitutes for the downstream element of the polyadenylation signal.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- A conserved 3'UTR short motif regulates gene expression in vertebrates.Nucleic acids research · 2026Article
- Transposon Regulation in thebioRxiv : the preprint server for biology · 2025Article
- Article
- Alternative Polyadenylation: a new frontier in post transcriptional regulation.Biomarker research · 2020Review
- Genome-wide profiling reveals alternative polyadenylation of mRNA in human non-small cell lung cancer.Journal of translational medicine · 2019Article
- GraphProt: modeling binding preferences of RNA-binding proteins.Genome biology · 2014Article
- The regulation of AβPP expression by RNA-binding proteins.Ageing research reviews · 2012Review
- The Ebola virus VP24 protein prevents hnRNP C1/C2 binding to karyopherin α1 and partially alters its nuclear import.The Journal of infectious diseases · 2011Article
- Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps.Virology journal · 2007Article
- Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers.The Biochemical journal · 2007Article
- A multispecies comparison of the metazoan 3'-processing downstream elements and the CstF-64 RNA recognition motif.BMC genomics · 2006Article
- A 57-nucleotide upstream early polyadenylation element in human papillomavirus type 16 interacts with hFip1, CstF-64, hnRNP C1/C2, and polypyrimidine tract binding protein.Journal of virology · 2005Article
- Alternative polyadenylation of cyclooxygenase-2.Nucleic acids research · 2005Article
- Secondary structure as a functional feature in the downstream region of mammalian polyadenylation signals.Molecular and cellular biology · 2004Article
- Identification of S1 proteins B2, C1 and D1 as AUF1 isoforms and their major role as heterogeneous nuclear ribonucleoprotein proteins.The Biochemical journal · 2003Article
- Downstream elements of mammalian pre-mRNA polyadenylation signals: primary, secondary and higher-order structures.Nucleic acids research · 2003Review
- The internal ribosome entry site-mediated translation of antiapoptotic protein XIAP is modulated by the heterogeneous nuclear ribonucleoproteins C1 and C2.Molecular and cellular biology · 2003Article
- Characterization of specific protein-RNA complexes associated with the coupling of polyadenylation and last-intron removal.Molecular and cellular biology · 2002Article
- Heterogeneous nuclear ribonucleoproteins C1 and C2 associate with the RNA component of human telomerase.Molecular and cellular biology · 2000Article
- Isolation and characterization of polyadenylation complexes assembled in vitro.RNA (New York, N.Y.) · 2000Article
1 more citing papers are in PubMed but not listed here.
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Abstract
Every RNA added to an in vitro polyadenylation extract became stably associated with both the heterogeneous nuclear ribonucleoprotein (hnRNP) A and C proteins, as assayed by immunoprecipitation analysis using specific monoclonal antibodies. UV-cross-linking analysis, however, which assays the specific spatial relationship of certain amino acids and RNA bases, indicated that the hnRNP C proteins, but not the A proteins, were associated with downstream sequences of the simian virus 40 late polyadenylation signal in a sequence-mediated manner. A tract of five consecutive uridylate residues was required for this interaction. The insertion of a five-base U tract into a pGEM4 polylinker-derived transcript was sufficient to direct sequence-specific cross-linking of the C proteins to RNA. Finally, the five-base uridylate tract restored efficient in vitro processing to several independent poly(A) signals in which it substituted for downstream element sequences. The role of the downstream element in polyadenylation efficiency, therefore, may be mediated by sequence-directed alignment or phasing of an hnRNP complex.
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