Evidence map›Paper›PMID 17008993›Full record

ArticlePlanta2007

PpRT1: the first complete gypsy-like retrotransposon isolated in Pinus pinaster.

Margarida Rocheta, Jorge Cordeiro, M Oliveira, Célia Miguel

Abstract read
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In one paragraph

Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 31 citations in OpenAlex.

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  6. Genome merger: from sequence rearrangements in triticale to their elimination in wheat-rye addition lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Margarida RochetaForest Biotech Lab, Instituto de Biologia Experimental e Tecnológica (IBET/ITQB), Quinta do Marquês, 2784-505 Oeiras, Portugal. rocheta@itqb.unl.pt
Jorge Cordeiro
M Oliveira
Célia Miguel
Instituto de Biologia Experimental e Tecnológica · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have isolated and characterized a complete retrotransposon sequence, named PpRT1, from the genome of Pinus pinaster. PpRT1 is 5,966 bp long and is closely related to IFG7 gypsy retrotransposon from Pinus radiata. The long terminal repeats (LTRs) have 333 bp each and show a 5.4% sequence divergence between them. In addition to the characteristic polypurine tract (PPT) and the primer binding site (PBS), PpRT1 carries internal regions with homology to retroviral genes gag and pol. The pol region contains sequence motifs related to the enzymes protease, reverse transcriptase, RNAseH and integrase in the same typical order known for Ty3/gypsy-like retrotransposons. PpRT1 was extended from an EST database sequence indicating that its transcription is occurring in pine tissues. Southern blot analyses indicate however, that PpRT1 is present in a unique or a low number of copies in the P. pinaster genome. The differences in nucleotide sequence found between PpRT1 and IFG7 may explain the strikingly different copy number in the two pine species genome. Based on the homologies observed when comparing LTR region among different gypsy elements we propose that the highly conserved LTR regions may be useful to amplify other retrotransposon sequences of the same or close retrotransposon family.

Indexed as

Genome, PlantAmino Acid SequenceBase SequenceBlotting, SouthernDNA, PlantModels, GeneticMolecular Sequence DataPhylogenyPinusRetroelementsRNA-Directed DNA PolymeraseSequence AlignmentSequence Analysis, DNATerminal Repeat SequencesDNA, PlantRetroelementsRNA-Directed DNA Polymerase

Identifiers

PMID17008993
OpenAlexW1966032152

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.