ArticleAnnals of botany2006
Interaction between methyl CpG-binding protein and ran GTPase during cell division in tobacco cultured cells.
Article in Annals of botany, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Roles of methyl-CpG-binding-domain (MBD) protiens in regulation of biological processes in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- MBD3 Regulates Male Germ Cell Division and Sperm Fertility inPlants (Basel, Switzerland) · 2023Article
- Genome-wide identification of MBD gene family members in Eleutherococcus senticosus with their expression motifs under drought stress and DNA demethylation.BMC genomics · 2023Article
- mFrontiers in genetics · 2022Article
- Proteome Analysis of Condensed Barley Mitotic Chromosomes.Frontiers in plant science · 2021Article
- Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles.Frontiers in plant science · 2021Article
- AtMBD4: A methylated DNA binding protein negatively regulates a subset of phosphate starvation genes.Journal of biosciences · 2019Article
- Genome-wide analysis of genes encoding MBD domain-containing proteins from tomato suggest their role in fruit development and abiotic stress responses.Molecular biology reports · 2018Article
- AtMBD6, a methyl CpG binding domain protein, maintains gene silencing in Arabidopsis by interacting with RNA binding proteins.Journal of biosciences · 2017Article
- The Small G Protein AtRAN1 Regulates Vegetative Growth and Stress Tolerance in Arabidopsis thaliana.PloS one · 2016Article
- Seed dormancy cycling in Arabidopsis: chromatin remodelling and regulation of DOG1 in response to seasonal environmental signals.The Plant journal : for cell and molecular biology · 2015Article
- Proteomic analysis of tomato (Lycopersicum esculentum var. cerasifarm) expressing the HBsAg gene by 2-dimensional difference gel electrophoresis.Plant foods for human nutrition (Dordrecht, Netherlands) · 2013Article
- The interaction of banana MADS-box protein MuMADS1 and ubiquitin-activating enzyme E-MuUBA in post-harvest banana fruit.Plant cell reports · 2013Article
- Mechanisms of plant spindle formation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- The Arabidopsis nuclear pore and nuclear envelope.The arabidopsis book · 2010Article
- A plasma-membrane linker for the phosphoinositide-specific phospholipase C in tobacco plants.Plant signaling & behavior · 2009Article
- Article
- Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.Annual review of biophysics · 2008Review
- The analysis of protein-protein interactions in plants by bimolecular fluorescence complementation.Plant physiology · 2007Review
- Perinuclear and nuclear envelope localizations of Arabidopsis Ran proteins.Plant cell reports · 2007Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsMethyl CpG-binding proteins are considered to play critical roles in epigenetic control of gene expression by recognizing and interacting with 5-methylcytosine (m(5)C) in eukaryotes. However, among 13 corresponding genes in Arabidopsis thaliana, designated as featuring a methyl-binding domain (MBD), only four have so far been shown actually to bind to m(5)C. One example, AtMBD5, was selected here to screen for interacting proteins.
methodsYeast two-hybrid assays were used for screening, and physical interaction was confirmed by pull-down and bimolecular fluorescence complementation (BiFC) assays. Cellular localization was analysed by fluorescence-tagged fusion proteins using tobacco (Nicotiana tabacum) cultured bright yellow 2 cells. KEY
resultsA gene finally identified was found to encode AtRAN3, a protein that belongs to the Ran GTPase family, which plays a critical role in nucleocytoplasmic transport and spindle bipolarization during cell division. AtMBD5 and AtRAN3 were clearly shown to interact in the nucleus by BiFC. On co-expression of AtMBD5-cyan fluorescence protein and yellow fluorescence protein-AtRAN3 in tobacco cells, both localized to the nucleus in the resting stage, migrating to the cytoplasm, primarily around chromatin, during mitosis, particularly at metaphase.
conclusionsThese results suggest that AtMBD5 becomes localized to the vicinity of chromosomes with the aid of AtRAN3 during cell division, and may play an important role not only in maintenance of chromatin structures by binding to m(5)C, but also in progress through mitosis by detaching from m(5)C. The present findings also shed light on the physiological function of Ran GTPases, direct target proteins of which have not thus far been well defined, suggesting their key role in chromatin movements in plant cells.
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