ArticleWorld journal of gastroenterology2006
Hemoglobin induces colon cancer cell proliferation by release of reactive oxygen species.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- The Role of Reactive Oxygen Species in Colorectal Cancer Initiation and Progression: Perspectives on Theranostic Approaches.Cancers · 2025Review
- The combined anticancer of peanut skin procyanidins and resveratrol to CACO-2 colorectal cancer cells.Food science & nutrition · 2023Article
- Preoperative Radiotherapy Leads to Significant Differences in the Plasma Protein Profile of Rectal Cancer Patients.Oncology · 2020Article
- Redox Imbalance in the Development of Colorectal Cancer.Journal of Cancer · 2017Review
- Cancer drug resistance: redox resetting renders a way.Oncotarget · 2016Review
- Article
- Influence of chronic moderate sleep restriction and exercise on inflammation and carcinogenesis in mice.Brain, behavior, and immunity · 2012Article
- Improved formulation of liposome-encapsulated hemoglobin with an anionic non-phospholipid.Colloids and surfaces. B, Biointerfaces · 2010Article
- Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration.Human genomics and proteomics : HGP · 2009Article
- Reactive oxygen species: a double-edged sword in oncogenesis.World journal of gastroenterology · 2009Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study whether hemoglobin could amplify colon cancer cell proliferation via reactive oxygen species (ROS) production.
methodsColon cancer cell line HT-29 was grown in the conventional method using RPMI1640 media. The viability of the cells was measured using the colorimetric MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay after adding hemoglobin. We determined reactive oxygen species levels to be indicators of oxidative stress in HT 29 cell lines with and without hemoglobin and/or 5-fluorouracil (5-FU), 5'-deoxy-5-fluorouridine (5-DFUR) using fluorometric dichlorofluorescin diacetate (DCFH-DA) assay.
resultsCellular proliferation was increased with hemoglobin in a concentration-dependent manner. A significant increment on ROS levels was found in HT 29 cells following hemoglobin incubation. The cytotoxic effects of 5-FU and 5-DFUR were significantly blunted by administration of hemoglobin. There was a slight increase of peroxiredoxin 1, superoxide dismutase 1 concentration according to different hemoglobin concentrations.
conclusionHemoglobin has a cellular proliferative effect on HT-29 colon cancer cell line by production of ROS. Also, hemoglobin abates cytotoxic effects of chemotherapeutic agents such as 5-FU and 5-DFUR.
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