ArticleAnalytical and bioanalytical chemistry2006
Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid.
Article in Analytical and bioanalytical chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Development of Magnetic Lateral Flow and Direct Competitive Immunoassays for Sensitive and Specific Detection of Halosulfuron-Methyl Using a Novel Hapten and Monoclonal Antibody.Foods (Basel, Switzerland) · 2023Article
- Uncovering a Phenomenon of Active Hormone Transcriptional Regulation during Early Somatic Embryogenesis inInternational journal of molecular sciences · 2022Article
- Design and Characterization of a Novel Hapten and Preparation of Monoclonal Antibody for Detecting Atrazine.Foods (Basel, Switzerland) · 2022Article
- Green synthesis and characterization of gold nanoparticles and their application for the rapid detection of glycyrrhizin with immunochromatographic strips.RSC advances · 2021Article
- Establishment of indirect competitive enzyme-linked immunosorbent assay for the detection of platycodin D in Radix Platycodonis.Journal of food and drug analysis · 2020Article
- Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein inInternational journal of analytical chemistry · 2020Article
- Biosynthesis and Signal Transduction of ABA, JA, and BRs in Response to Drought Stress of Kentucky Bluegrass.International journal of molecular sciences · 2019Article
- Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick.Analytical and bioanalytical chemistry · 2016Article
- Development of a Specific Monoclonal Antibody for the Quantification of Artemisinin in Artemisia annua and Rat Serum.Analytical chemistry · 2016Article
- Article
- Highly sensitive nanomechanical assay for the stress transmission of carbon chain.Sensors and actuators. B, Chemical · 2013Article
- Development of a specific monoclonal antibody-based ELISA to measure the artemether content of antimalarial drugs.PloS one · 2013Article
- Effect of water deficit on biomass production and accumulation of secondary metabolites in roots ofRussian journal of plant physiology: a comprehensive Russian journal on modern phytophysiology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycyrrhizic acid (GL) is a major active compound of licorice. The specific monoclonal antibody (MAb) (designated as 8F8A8H42H7) against GL was produced with the immunogen GL-BSA conjugate. The dissociation constant (Kd) value of the MAb was approximately 9.96x10(-10) M. The cross reactivity of the MAb with glycyrrhetic acid was approximately 2.6%. The conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA adapted with a modified procedure were established using the MAb. The IC50 value and the detect range by the conventional icELISA were 1.1 ng mL-1 and 0.2-5.1 ng mL-1, respectively. The IC50 value and the detect range by the simplified icELISA were 5.3 ng mL-1 and 1.2-23.8 ng mL-1, respectively. The two icELISA formats were used to analyze GL contents in the roots of wild licorice and different parts of cultivated licorice (Glycyrrhiza uralensis Fisch). The results obtained with the two icELISAs agreed well with those of the HPLC analysis. The correlation coefficient was more than 0.98 between HPLC and the two icELISAs. The two icELISAs were shown to be appropriate, simple, and effective for the quality control of raw licorice root materials.
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