ArticleThe EMBO journal2006
Tissue kallikrein stimulates Ca(2+) reabsorption via PKC-dependent plasma membrane accumulation of TRPV5.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 81 citations in OpenAlex.
- An update on regulation of the polymodal TRPV4 channel by protein phosphorylation.Channels (Austin, Tex.) · 2026Review
- Klotho in the kidney distal convolution regulates urinary Klotho excretion and kidney calcium reabsorption, but not phosphate homeostasis.Kidney international · 2026Article
- Repeated stress to the skin amplifies neutrophil infiltration in a keratin 17- and PKCα-dependent manner.PLoS biology · 2024Article
- The ion channel TRPV5 regulates B-cell signaling and activation.Frontiers in immunology · 2024Article
- The importance of kidney calcium handling in the homeostasis of extracellular fluid calcium.Pflugers Archiv : European journal of physiology · 2022Review
- Article
- TRPM8 and prostate: a cold case?Pflugers Archiv : European journal of physiology · 2018Review
- Modulation of renal calcium and phosphate transporting proteins by dietary nitrogen and/or calcium in young goats.Journal of animal science · 2018Article
- Interspecies differences in PTH-mediated PKA phosphorylation of the epithelial calcium channel TRPV5.Pflugers Archiv : European journal of physiology · 2017Article
- TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.The Journal of cell biology · 2015Article
- Distal convoluted tubule.Comprehensive Physiology · 2015Review
- Coordinated regulation of TRPV5-mediated Ca²⁺ transport in primary distal convolution cultures.Pflugers Archiv : European journal of physiology · 2014Article
- Reduced renal calcium excretion in the absence of sclerostin expression: evidence for a novel calcium-regulating bone kidney axis.Journal of the American Society of Nephrology : JASN · 2014Review
- β1-Adrenergic receptor signaling activates the epithelial calcium channel, transient receptor potential vanilloid type 5 (TRPV5), via the protein kinase A pathway.The Journal of biological chemistry · 2014Article
- Regulation of the epithelial Ca²⁺ channel TRPV5 by reversible histidine phosphorylation mediated by NDPK-B and PHPT1.Molecular biology of the cell · 2014Article
- Proteomic characterization of serine hydrolase activity and composition in normal urine.Clinical proteomics · 2013Article
- Autosomal dominant hypercalciuria in a mouse model due to a mutation of the epithelial calcium channel, TRPV5.PloS one · 2013Article
- Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria.Journal of the American Society of Nephrology : JASN · 2012Article
- Tissue kallikrein activation of the epithelial Na channel.American journal of physiology. Renal physiology · 2012Article
- A new look at electrolyte transport in the distal tubule.Annual review of physiology · 2012Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transient receptor potential vanilloid 5 (TRPV5) channel determines urinary Ca(2+) excretion, and is therefore critical for Ca(2+) homeostasis. Interestingly, mice lacking the serine protease tissue kallikrein (TK) exhibit robust hypercalciuria comparable to the Ca(2+) leak in TRPV5 knockout mice. Here, we delineated the molecular mechanism through which TK stimulates Ca(2+) reabsorption. Using TRPV5-expressing primary cultures of renal Ca(2+)-transporting epithelial cells, we showed that TK activates Ca(2+) reabsorption. The stimulatory effect of TK was mimicked by bradykinin (BK) and could be reversed by application of JE049, a BK receptor type 2 antagonist. A cell permeable analog of DAG increased TRPV5 activity within 30 min via protein kinase C activation of the channel since mutation of TRPV5 at the putative PKC phosphorylation sites S299 and S654 prevented the stimulatory effect of TK. Cell surface labeling revealed that TK enhances the amount of wild-type TRPV5 channels, but not of the TRPV5 S299A and S654A mutants, at the plasma membrane by delaying its retrieval. In conclusion, TK stimulates Ca(2+) reabsorption via the BK-activated PLC/DAG/PKC pathway and the subsequent stabilization of the TRPV5 channel at the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.