ArticleThe EMBO journal2006
Restraining the conformation of HIV-1 gp120 by removing a flexible loop.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Stapled HIV-1 peptides recapitulate antigenic structures and engage broadly neutralizing antibodies.Nature structural & molecular biology · 2014Article
- Increased functional stability and homogeneity of viral envelope spikes through directed evolution.PLoS pathogens · 2013Article
- HIV-1 Env gp120 structural determinants for peptide triazole dual receptor site antagonism.Proteins · 2013Article
- Lineage-specific differences between human and simian immunodeficiency virus regulation of gp120 trimer association and CD4 binding.Journal of virology · 2012Article
- Direct antibody access to the HIV-1 membrane-proximal external region positively correlates with neutralization sensitivity.Journal of virology · 2011Article
- Quaternary structures of HIV Env immunogen exhibit conformational vicissitudes and interface diminution elicited by ligand binding.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Conformational and structural features of HIV-1 gp120 underlying the dual receptor antagonism by cross-reactive neutralizing antibody m18.Biochemistry · 2011Article
- Measuring HIV fusion mediated by envelopes from primary viral isolates.Methods (San Diego, Calif.) · 2011Article
- Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody.Structure (London, England : 1993) · 2010Article
- Structural basis for broad and potent neutralization of HIV-1 by antibody VRC01.Science (New York, N.Y.) · 2010Article
- Topological layers in the HIV-1 gp120 inner domain regulate gp41 interaction and CD4-triggered conformational transitions.Molecular cell · 2010Article
- HIV evolution in early infection: selection pressures, patterns of insertion and deletion, and the impact of APOBEC.PLoS pathogens · 2009Article
- A fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The trimeric HIV/SIV envelope glycoprotein, gp160, is cleaved to noncovalently associated fragments, gp120 and gp41. Binding of gp120 to viral receptors leads to large structural rearrangements in both fragments. The unliganded gp120 core has a disordered beta3-beta5 loop, which reconfigures upon CD4 binding into an ordered, extended strand. Molecular modeling suggests that residues in this loop may contact gp41. We show here that deletions in the beta3-beta5 loop of HIV-1 gp120 weaken the binding of CD4 and prevent formation of the epitope for monoclonal antibody (mAb) 17b (which recognizes the coreceptor site). Formation of an encounter complex with CD4 binding and interactions of gp120 with mAbs b12 and 2G12 are not affected by these deletions. Thus, deleting the beta3-beta5 loop blocks the gp120 conformational change and may offer a strategy for design of restrained immunogens. Moreover, mutations in the SIV beta3-beta5 loop lead to greater spontaneous dissociation of gp120 from cell-associated trimers. We suggest that the CD4-induced rearrangement of this loop releases structural constraints on gp41 and thus potentiates its fusion activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.