ArticleMolecular biology of the cell2006
TIF1 activates the intra-S-phase checkpoint response in the diploid micronucleus and amitotic polyploid macronucleus of Tetrahymena.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Developmentally Programmed Switches in DNA Replication: Gene Amplification and Genome-Wide Endoreplication inMicroorganisms · 2023Article
- Transcriptome analysis of the binucleate ciliateMolecular biology of the cell · 2023Article
- Disruption of a ∼23-24 nucleotide small RNA pathway elevates DNA damage responses inMolecular biology of the cell · 2021Article
- An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells.Frontiers in cell and developmental biology · 2021Article
- A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation.Nucleic acids research · 2018Article
- Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.PLoS genetics · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.Genes & development · 2013Article
- MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena.Chromosoma · 2010Article
- Crystal structures of DNA-Whirly complexes and their role in Arabidopsis organelle genome repair.The Plant cell · 2010Article
- Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage.Molecular biology of the cell · 2009Article
- Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.The EMBO journal · 2009Article
- Overexpression of mtDNA-associated AtWhy2 compromises mitochondrial function.BMC plant biology · 2008Article
- Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition.The EMBO journal · 2007Article
- Tetrahymena POT1a regulates telomere length and prevents activation of a cell cycle checkpoint.Molecular and cellular biology · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ribosomal DNA origin binding protein Tif1p regulates the timing of rDNA replication and is required globally for proper S-phase progression and division of the Tetrahymena thermophila macronucleus. Here, we show that Tif1p safeguards chromosomes from DNA damage in the mitotic micronucleus and amitotic macronucleus. TIF1p localization is dynamically regulated as it moves into the micro- and macronucleus during the respective S phases. TIF1 disruption mutants are hypersensitive to hydroxyurea and methylmethanesulfonate, inducers of DNA damage and intra-S-phase checkpoint arrest in all examined eukaryotes. TIF1 mutants incur double-strand breaks in the absence of exogenous genotoxic stress, destabilizing all five micronuclear chromosomes. Wild-type Tetrahymena elicits an intra-S-phase checkpoint response that is induced by hydroxyurea and suppressed by caffeine, an inhibitor of the apical checkpoint kinase ATR/MEC1. In contrast, hydroxyurea-challenged TIF1 mutants fail to arrest in S phase or exhibit caffeine-sensitive Rad51 overexpression, indicating the involvement of TIF1 in checkpoint activation. Although aberrant micro- and macronuclear division occurs in TIF1 mutants and caffeine-treated wild-type cells, TIF1p bears no similarity to ATR or its substrates. We propose that TIF1 and ATR function in the same epistatic pathway to regulate checkpoint responses in the diploid mitotic micronucleus and polyploid amitotic macronucleus.
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