ArticleMolecular biology of the cell2006
Not all secretory granules are created equal: Partitioning of soluble content proteins.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed.
- Molecular requirements of chromogranin B for the long-sought anion shunter of regulated secretion.International journal of biological macromolecules · 2025Article
- Protein aggregates and biomolecular condensates: implications for human health and disease.Frontiers in molecular biosciences · 2025Review
- Chromogranin B (CHGB) is dimorphic and responsible for dominant anion channels delivered to cell surface via regulated secretion.Frontiers in molecular neuroscience · 2023Article
- Growth Hormone(s), Testosterone, Insulin-Like Growth Factors, and Cortisol: Roles and Integration for Cellular Development and Growth With Exercise.Frontiers in endocrinology · 2020Review
- Salvianolic acid A inhibits tumor-associated angiogenesis by blocking GRP78 secretion.Naunyn-Schmiedeberg's archives of pharmacology · 2019Article
- Platelet biology and functions: new concepts and clinical perspectives.Nature reviews. Cardiology · 2019Review
- Alternate promoter usage generates two subpopulations of the neuronal RhoGEF Kalirin-7.Journal of neurochemistry · 2017Article
- Copper, zinc and calcium: imaging and quantification in anterior pituitary secretory granules.Metallomics : integrated biometal science · 2016Article
- Polymers in the gut compress the colonic mucus hydrogel.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.PLoS genetics · 2016Article
- Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity.Journal of cell science · 2016Article
- Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets.Diabetologia · 2015Article
- An N-terminal Amphipathic Helix Binds Phosphoinositides and Enhances Kalirin Sec14 Domain-mediated Membrane Interactions.The Journal of biological chemistry · 2015Article
- Quantitative analysis of mouse pancreatic islet architecture by serial block-face SEM.Journal of structural biology · 2015Article
- AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.Traffic (Copenhagen, Denmark) · 2014Article
- A histidine-rich linker region in peptidylglycine α-amidating monooxygenase has the properties of a pH sensor.The Journal of biological chemistry · 2014Article
- ProSAAS-derived peptides are differentially processed and sorted in mouse brain and AtT-20 cells.PloS one · 2014Article
- Spinal astrocytes produce and secrete dynorphin neuropeptides.Neuropeptides · 2013Article
- Article
- Lumenal loop M672-P707 of the Menkes protein (ATP7A) transfers copper to peptidylglycine monooxygenase.Journal of the American Chemical Society · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Secretory granules carrying fluorescent cargo proteins are widely used to study granule biogenesis, maturation, and regulated exocytosis. We fused the soluble secretory protein peptidylglycine alpha-hydroxylating monooxygenase (PHM) to green fluorescent protein (GFP) to study granule formation. When expressed in AtT-20 or GH3 cells, the PHM-GFP fusion protein partitioned from endogenous hormone (adrenocorticotropic hormone, growth hormone) into separate secretory granule pools. Both exogenous and endogenous granule proteins were stored and released in response to secretagogue. Importantly, we found that segregation of content proteins is not an artifact of overexpression nor peculiar to GFP-tagged proteins. Neither luminal acidification nor cholesterol-rich membrane microdomains play essential roles in soluble content protein segregation. Our data suggest that intrinsic biophysical properties of cargo proteins govern their differential sorting, with segregation occurring during the process of granule maturation. Proteins that can self-aggregate are likely to partition into separate granules, which can accommodate only a few thousand copies of any content protein; proteins that lack tertiary structure are more likely to distribute homogeneously into secretory granules. Therefore, a simple "self-aggregation default" theory may explain the little acknowledged, but commonly observed, tendency for both naturally occurring and exogenous content proteins to segregate from each other into distinct secretory granules.
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Registered trials
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