ArticleAntimicrobial agents and chemotherapy2006
The mef(E)-carrying genetic element (mega) of Streptococcus pneumoniae: insertion sites and association with other genetic elements.
Article in Antimicrobial agents and chemotherapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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45 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Serotype replacement and mobile genetic elements inMicrobial genomics · 2025Pooled it
- Phylogenetic analysis of serotype 19A-sequence type (ST)2331Microbial genomics · 2026Article
- Large-scale global molecular epidemiology of antibiotic resistance determinants inMicrobial genomics · 2025Article
- Ultrastructural, metabolic and genetic characteristics of determinants facilitating the acquisition of macrolide resistance by Streptococcus pneumoniae.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2024Article
- The Crisis of Macrolide Resistance in Pneumococci in Latin America.The American journal of tropical medicine and hygiene · 2024Review
- Convergent impact of vaccination and antibiotic pressures on pneumococcal populations.Cell chemical biology · 2024Review
- TheJournal of bacteriology · 2023Article
- Regulation of the macrolide resistance ABC-F translation factor MsrD.Nature communications · 2023Article
- The Molecular Epidemiology of Pneumococcal Strains Isolated from the Nasopharynx of Preschool Children 3 Years after the Introduction of the PCV Vaccination Program in Poland.International journal of molecular sciences · 2023Article
- Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)-msr(D) macrolide efflux system in Streptococcus pneumoniae.BMC research notes · 2021Article
- The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci.eLife · 2021Article
- WGS of CommensalPathogens (Basel, Switzerland) · 2021Article
- Genomics and Genetics ofMicrobiology spectrum · 2019Review
- High Frequency of Macrolide-ResistantTanaffos · 2019Article
- High-Level Macrolide Resistance Due to the Mega Element [Frontiers in microbiology · 2019Article
- Type M Resistance to Macrolides Is Due to a Two-Gene Efflux Transport System of the ATP-Binding Cassette (ABC) Superfamily.Frontiers in microbiology · 2018Article
- Complete Genome Sequences of Three Multidrug-Resistant Clinical Isolates ofGenome announcements · 2017Article
- Article
- Resistance to Macrolide Antibiotics in Public Health Pathogens.Cold Spring Harbor perspectives in medicine · 2016Review
- Macrolide Resistance inFrontiers in cellular and infection microbiology · 2016Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure of the macrolide efflux genetic assembly (mega) element, its genomic locations, and its association with other resistance determinants and genetic elements were investigated in 16 Streptococcus pneumoniae isolates carrying mef(E), of which 1 isolate also carried tet(M) and 4 isolates also carried tet(M) and erm(B). All isolates carried a mega element of similar size and structure that included the operon mef(E)-msr(D) encoding the efflux transport system. Among tetracycline-susceptible isolates, six different integration sites were identified, five of which were recognized inside open reading frames present in the R6 genome. In the five isolates also carrying tet(M), mega was inserted in different genetic contexts. In one isolate, it was part of previously described Tn916-like element Tn2009. In another isolate, mega was inserted in a transposon similar to Tn2009 that also included an erm(B) element. This new composite transposon was designated Tn2010. Neither Tn2009 nor Tn2010 could be transferred by conjugation to pneumococcal or enterococcal recipients. In the three isolates in which mega was not physically linked with tet(M), this gene was associated with erm(B) in transposon Tn3872, a Tn916-like element. Homologies between the chromosomal insertions of these composite transposons and sequences of multidrug-resistant pneumococcal genomes in the databases indicate the presence of preferential sites for the integration of composite Tn916-like elements carrying multiple resistance determinants in S. pneumoniae.
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