Evidence map›Paper›PMID 17005760›Full record

ArticleJournal of clinical microbiology2006

Simultaneous amplification and identification of 25 human papillomavirus types with Templex technology.

Jian Han, David C Swan, Sharon J Smith, Shanjuan H Lum, Susan E Sefers, Elizabeth R Unger, Yi-Wei Tang

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Increased risk of noninfluenza respiratory virus infections associated with receipt of inactivated influenza vaccine.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012
    Trial
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  9. The human Vδ2Nature communications · 2018
    Article
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  14. Diversity and divergence of the glioma-infiltrating T-cell receptor repertoire.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Viral surveillance and discovery.Current opinion in virology · 2013
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jian HanGenaco Biomedical Products, Inc., Huntsville, Alabama 35805, USA.
David C Swan
Sharon J Smith
Shanjuan H Lum
Susan E Sefers
Elizabeth R Unger
Yi-Wei Tang

Funding

High Throughput Method for Cancer related HPV subtypingR43CA106061 · NCI · GENACO BIOMEDICAL PRODUCTS, INC. · PI HAN, JIAN · 2004 to 2004
$100k
NCI NIH HHS R43 CA106061NCI NIH HHS R43-CA106061-01
6 · The paper itself

Abstract

The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E7 genes. The analytical sensitivities of the Templex assay for HPV type 16 (HPV-16), -18, and -56 were 20, 100, and 20 copies per reaction mixture, respectively. The Templex assay provides semiquantitative information on each type when multiple HPV types coexist in one reaction. We tested 109 clinical cervical specimens previously evaluated with the Digene HC2 high-risk HPV DNA test and found 95.4% concordance between the assay results. The Templex assay provided type-specific results and found multiple types in 29.2% (14 of 48) of high-risk HPV-positive samples. The entire Templex procedure, including DNA extraction, can be completed within 5 hours, providing a rapid and reliable diagnostic tool for HPV detection and typing that is amenable to automation.

Indexed as

Cervix UteriFemaleGenotypeHuman papillomavirus 16Human papillomavirus 18HumansNucleic Acid Amplification TechniquesPapillomaviridaePlasmidsPolymerase Chain Reaction

Identifiers

PMID17005760
PMCPMC1698316

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.