ArticleJournal of clinical microbiology2006
Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 658 papers, 3 of them syntheses that pooled it.
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Who cites it
658 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Quantifying within-household tuberculosis transmission: a systematic review and prospective cohort study.International journal of epidemiology · 2026Pooled it
- Mycobacterium tuberculosis mixed infections and drug resistance in sub-Saharan Africa: a systematic review.African health sciences · 2022Pooled it
- Methods for Detecting Mycobacterial Mixed Strain Infections-A Systematic Review.Frontiers in genetics · 2020Pooled it
- Trial
- Genomic epidemiology of Mycobacterium tuberculosis in Santa Catarina, Southern Brazil.Scientific reports · 2020Trial
- Trial
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- A Case of Transmission of Bedaquiline- and Linezolid-Resistant Tuberculosis.International journal of molecular sciences · 2026Article
- Molecular Characterization ofHealth science reports · 2026Article
- Key impact of Beijing strains including new resistant clusters on spread of multidrug-resistant tuberculosis in northern Russia.Microbiology spectrum · 2026Article
- Understanding recurrence inJournal of clinical microbiology · 2026Review
- Insight into the genetic diversity ofMicrobiology spectrum · 2026Article
- Long-term refined genomic analysis of tuberculosis clusters to distinguish between ongoing transmission, reactivations or diagnostic delays, Almería, Spain, 2003 to 2024.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2026Article
- Spatial clustering and genetic diversity of Mycobacterium tuberculosis and associated diagnostic delays in Nairobi County, Kenya.BMC infectious diseases · 2026Article
- Genetic Diversity and Molecular Epidemiology ofPathogens (Basel, Switzerland) · 2026Article
- Study on the species spectrum, drug resistance spectrum and molecular characteristics of clinicalFrontiers in microbiology · 2026Article
- Temporal and regional dynamics of Mycobacterium tuberculosis in Oman.Scientific reports · 2025Article
- Evaluation of thermal desorption-electrospray ionization mass spectrometry for characterization ofJournal of mass spectrometry and advances in the clinical lab · 2025Article
- Genomic characterization of XDR Mycobacterium tuberculosis isolates in Argentina (2006-2015).BMC infectious diseases · 2025Article
- Molecular epidemiology of tuberculosis in Western Burkina Faso: insights from a MIRU-VNTR study.BMC microbiology · 2025Article
598 more citing papers are in PubMed but not listed here.
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23 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular typing based on 12 loci containing variable numbers of tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTRs) has been adopted in combination with spoligotyping as the basis for large-scale, high-throughput genotyping of Mycobacterium tuberculosis. However, even the combination of these two methods is still less discriminatory than IS6110 fingerprinting. Here, we define an optimized set of MIRU-VNTR loci with a significantly higher discriminatory power. The resolution and the stability/robustness of 29 loci were analyzed, using a total of 824 tubercle bacillus isolates, including representatives of the main lineages identified worldwide so far. Five loci were excluded for lack of robustness and/or stability in serial isolates or isolates from epidemiologically linked patients. The use of the 24 remaining loci increased the number of types by 40%--and by 23% in combination with spoligotyping--among isolates from cosmopolitan origins, compared to those obtained with the original set of 12 loci. Consequently, the clustering rate was decreased by fourfold--by threefold in combination with spoligotyping--under the same conditions. A discriminatory subset of 15 loci with the highest evolutionary rates was then defined that concentrated 96% of the total resolution obtained with the full 24-locus set. Its predictive value for evaluating M. tuberculosis transmission was found to be equal to that of IS6110 restriction fragment length polymorphism typing, as shown in a companion population-based study. This 15-locus system is therefore proposed as the new standard for routine epidemiological discrimination of M. tuberculosis isolates and the 24-locus system as a high-resolution tool for phylogenetic studies.
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