ArticleJournal of clinical microbiology2006
Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Comparative Description of Dermatophyte Genomes: A State-of-the-Art Review.Mycopathologia · 2023Pooled it
- Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia.Biomedica : revista del Instituto Nacional de Salud · 2022Article
- Investigating cross-contamination by yeast strains from dental solid waste to waste-handling workers by DNA sequencing.MicrobiologyOpen · 2018Article
- Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Kerion caused by Microsporum audouinii in a child.Medical mycology case reports · 2013Article
- Single-step PCR using (GACA)4 primer: utility for rapid identification of dermatophyte species and strains.Journal of clinical microbiology · 2008Article
- The unique probe selector: a comprehensive web service for probe design and oligonucleotide arrays.BMC bioinformatics · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.
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Registered trials
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