ArticlePlanta2007
Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 94 citations in OpenAlex.
- A Comprehensive Review on the Biosynthesis of Tropane Alkaloids.Molecules (Basel, Switzerland) · 2026Review
- A review on Mitragyna alkaloids pharmacology, biosynthesis and biotechnological insights.Journal, genetic engineering & biotechnology · 2026Review
- Investigation of genes involved in scent and color production in Rosa damascena Mill.Scientific reports · 2024Article
- Alteration in the callogenesis, tropane alkaloid formation, and gene expression in Hyoscyamus niger under clinorotation.Protoplasma · 2024Article
- Accumulation of Polyphenols and Associated Gene Expression in Hairy Roots ofInternational journal of molecular sciences · 2024Article
- Metabolic Effects of Elicitors on the Biosynthesis of Tropane Alkaloids in Medicinal Plants.Plants (Basel, Switzerland) · 2023Review
- The Light- and Jasmonic Acid-InducedInternational journal of molecular sciences · 2023Article
- Expression ofGenes · 2023Article
- Review
- Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants.Frontiers in plant science · 2023Review
- Exploring the Effect of Methyl Jasmonate on the Expression of microRNAs Involved in Biosynthesis of Active Compounds of Rosemary Cell Suspension Cultures through RNA-Sequencing.International journal of molecular sciences · 2022Article
- Unravelling the multi-faceted regulatory role of polyamines in plant biotechnology, transgenics and secondary metabolomics.Applied microbiology and biotechnology · 2022Review
- Article
- MicroRNAome Profile ofInternational journal of molecular sciences · 2019Article
- Genetically engineered hairy root cultures of Hyoscyamus senecionis and H. muticus: ploidy as a promising parameter in the metabolic engineering of tropane alkaloids.Plant cell reports · 2017Article
- Enhancing Tropane Alkaloid Production Based on the Functional Identification of Tropine-Forming Reductase inFrontiers in plant science · 2017Article
- Tropane and Granatane Alkaloid Biosynthesis: A Systematic Analysis.Molecules (Basel, Switzerland) · 2016Review
- Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare.Physiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories.Molecules (Basel, Switzerland) · 2016Review
- Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA from Nicotiana tabacum encoding Putrescine N-methyltransferase (PMT), which catalyzes the first committed step in the biosynthesis of tropane alkaloids, has been introduced into the genome of a scopolamine-producing Hyoscyamus niger mediated by the disarmed Agrobacterium tumefaciens strain C58C1, which also carries Agrobacterium rhizogenes Ri plasmid pRiA4, and expressed under the control of the CaMV 35S promoter. Hairy root lines transformed with pmt presented fivefold higher PMT activity than the control, and the methylputrescine (MPUT) levels of the resulting engineered hairy roots increased four to fivefold compared to the control and wild-type roots, but there was no significant increase in tropane alkaloids. However, after methyl jasmonate (MeJA) treatment, a considerable increase of PMTase and endogenous H6Hase as well as an increase in scopolamine content was found either in the transgenic hairy roots or the control. The results indicate that hairy root lines over-expressing pmt have a high capacity to synthesize MPUT, whereas their ability to convert hyoscyamine into scopolamine is very limited. Exposure to MeJA strongly stimulated both polyamine and tropane biosynthesis pathways and elicitation led to more or less enhanced production simultaneously.
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Registered trials
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