ArticleMolecular and cellular biology1990
RNA processing is a limiting step for murine tumor necrosis factor beta expression in response to interleukin-2.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- High frequency trans-splicing in a cell line producing spliced and polyadenylated RNA polymerase I transcripts from an rDNA-myc chimeric gene.Nucleic acids research · 2005Article
- Unr is required in vivo for efficient initiation of translation from the internal ribosome entry sites of both rhinovirus and poliovirus.Journal of virology · 2003Article
- In vivo kinetics of mRNA splicing and transport in mammalian cells.Molecular and cellular biology · 2002Article
- Mature mRNAs accumulated in the nucleus are neither the molecules in transit to the cytoplasm nor constitute a stockpile for gene expression.RNA (New York, N.Y.) · 2000Article
- A cis-acting element in the 3'-untranslated region of human TNF-alpha mRNA renders splicing dependent on the activation of protein kinase PKR.Genes & development · 1999Article
- Article
- 2-Aminopurine selectively inhibits splicing of tumor necrosis factor alpha mRNA.Molecular and cellular biology · 1996Article
- Large exon size does not limit splicing in vivo.Molecular and cellular biology · 1994Article
- Contrasted cis-acting effects of downstream 5' splice sites on the splicing of a retained intron: the adenoviral E1A pre-mRNA model.Nucleic acids research · 1993Article
- Order of intron removal during splicing of endogenous adenine phosphoribosyltransferase and dihydrofolate reductase pre-mRNA.Molecular and cellular biology · 1993Article
- A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant.The EMBO journal · 1992Article
- Chromatin structure and DNase I hypersensitivity in the transcriptionally active and inactive porcine tumor necrosis factor gene locus.Nucleic acids research · 1992Article
- Beta-globin nonsense mutation: deficient accumulation of mRNA occurs despite normal cytoplasmic stability.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Spontaneous deletions in Ig heavy chain genes: flanking sequences influence splice site selection.Nucleic acids research · 1991Article
- An analysis of vertebrate mRNA sequences: intimations of translational control.The Journal of cell biology · 1991Review
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1991Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously reported that tumor necrosis factor beta (TNF beta) expression is induced by interleukin-2 (IL-2) in the murine lymphocytic T-cell line CTLL-2. In this study, we have characterized the nuclear and cytoplasmic TNF beta transcript and assessed their role in TNF beta gene expression. A unique feature of TNF beta expression was the accumulation of nuclear precursors, which reflected a slow nuclear RNA processing. As a consequence, there was a delay in the appearance of cytoplasmic messengers after the transcriptional induction of TNF beta by IL-2. We also found that two messengers, the fully spliced messenger and an intron 3-retaining messenger, were exported to the cytoplasm and actively translated. The same pattern of expression was observed in concanavalin A-stimulated splenocytes, although the level of expression was much lower than in CTLL-2 cells. The simple genetic structure and the high level of accumulation of nuclear precursors make TNF beta a particularly attractive model system to use for studies of RNA processing and cytoplasmic transport of partially spliced messengers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.