ArticleBiochemistry2006
The vault exterior shell is a dynamic structure that allows incorporation of vault-associated proteins into its interior.
Article in Biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Structural flexibility of the human vault particle revealed by high-resolution cryo-EM and molecular dynamics simulations.Nature communications · 2026Article
- The vault associates with membranes in situ.Nature communications · 2026Article
- Structural insights into the roles of PARP4 and NADNature communications · 2025Article
- Vault Particles in Cancer Progression, Multidrug Resistance, and Drug Delivery: Current Insights and Future Applications.International journal of molecular sciences · 2025Review
- Structure, Dynamics and Functional Implications of the Eukaryotic Vault Complex.Sub-cellular biochemistry · 2024Review
- Manufacturing of non-viral protein nanocages for biotechnological and biomedical applications.Frontiers in bioengineering and biotechnology · 2023Review
- Article
- Review
- Vault RNAs: hidden gems in RNA and protein regulation.Cellular and molecular life sciences : CMLS · 2021Review
- Bioengineered recombinant vault nanoparticles coupled with NY-ESO-1 glioma-associated antigens induce maturation of native dendritic cells.Journal of neuro-oncology · 2020Article
- Latest Advances in the Development of Eukaryotic Vaults as Targeted Drug Delivery Systems.Pharmaceutics · 2019Review
- Review
- Solution Structures of Engineered Vault Particles.Structure (London, England : 1993) · 2018Article
- Modulation of the Vault Protein-Protein Interaction for Tuning of Molecular Release.Scientific reports · 2017Article
- Engineering protein nanocages as carriers for biomedical applications.NPG Asia materials · 2017Review
- Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities.Cold Spring Harbor perspectives in biology · 2016Review
- Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope.Vaccine · 2015Article
- Polyribosomes are molecular 3D nanoprinters that orchestrate the assembly of vault particles.ACS nano · 2014Article
- Bioengineered vaults: self-assembling protein shell-lipophilic core nanoparticles for drug delivery.ACS nano · 2014Article
- 3'-ethynylcytidine, an RNA polymerase inhibitor, combined with cisplatin exhibits a potent synergistic growth-inhibitory effect via Vaults dysfunction.BMC cancer · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Vaults are 13 million Da ribonucleoprotein particles with a highly conserved structure. Expression and assembly by multimerization of an estimated 96 copies of a single protein, termed the major vault protein (MVP), is sufficient to form the minimal structure and entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, VPARP and TEP1, and a small untranslated vault RNA are also associated with vaults. We used the Sf9 insect cell expression system to form MVP-only recombinant vaults and performed a series of protein-mixing experiments to test whether this particle shell is able to exclude exogenous proteins from interacting with the vault interior. Surprisingly, we found that VPARP and TEP1 are able to incorporate into vaults even after the formation of the MVP vault particle shell is complete. Electrospray molecular mobility analysis and spectroscopic studies of vault-interacting proteins were used to confirm this result. Our results demonstrate that the protein shell of the recombinant vault particle is a dynamic structure and suggest a possible mechanism for in vivo assembly of vault-interacting proteins into preformed vaults. Finally, this study suggests that the vault interior may functionally interact with the cellular milieu.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.