ArticleMolecular genetics and genomics : MGG2007
Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment.
Article in Molecular genetics and genomics : MGG, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- The establishment of CDK9/RNA PolII/H3K4me3/DNA methylation feedback promotes HOTAIR expression by RNA elongation enhancement in cancer.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.G3 (Bethesda, Md.) · 2021Article
- Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells.Nature communications · 2021Article
- Article
- Acetylation on histone H3 lysine 9 mediates a switch from transcription initiation to elongation.The Journal of biological chemistry · 2017Article
- Dormancy activation mechanism of tracheal stem cells.Oncotarget · 2016Article
- Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification.Nucleic acids research · 2015Article
- P-TEFb, the super elongation complex and mediator regulate a subset of non-paused genes during early Drosophila embryo development.PLoS genetics · 2015Article
- Chromatin modification by the RNA Polymerase II elongation complex.Transcription · 2014Review
- Role of noncoding RNAs in the regulation of P-TEFb availability and enzymatic activity.BioMed research international · 2014Review
- RNA polymerase II elongation control.Annual review of biochemistry · 2012Review
- Perspective of cyclin-dependent kinase 9 (CDK9) as a drug target.Current pharmaceutical design · 2012Review
- Phosphorylation of histone H1 by P-TEFb is a necessary step in skeletal muscle differentiation.Journal of cellular physiology · 2012Article
- The CDK9/cyclin T1 subunits of P-TEFb in mouse oocytes and preimplantation embryos: a possible role in embryonic genome activation.BMC developmental biology · 2011Article
- The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization.Nucleic acids research · 2011Article
- The TFIIB tip domain couples transcription initiation to events involved in RNA processing.The Journal of biological chemistry · 2010Article
- HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP.Molecular cell · 2010Article
- HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription.Molecular cell · 2010Article
- Histone H3 lysine 4 (H3K4) methylation in development and differentiation.Developmental biology · 2010Review
- AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia.Molecular cell · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Phosphorylation of the large RNA Polymerase II subunit C-terminal domain (CTD) is believed to be important in promoter clearance and for recruiting protein factors that function in messenger RNA synthesis and processing. P-TEFb is a protein kinase that targets the (CTD). The goal of this study was to identify chromatin modifications and associations that require P-TEFb activity in vivo. We knocked down the catalytic subunit of P-TEFb, Cdk9, in Drosophila melanogaster using RNA interference. Cdk9 knockdown flies die during metamorphosis. Phosphorylation at serine 2 and serine 5 of the CTD heptad repeat were both dramatically reduced in knockdown larvae. Hsp 70 mRNA induction by heat shock was attenuated in Cdk9 knockdown larvae. Both mono- and trimethylation of histone H3 at lysine 4 were dramatically reduced, suggesting a link between CTD phosphorylation and histone methylation in transcribed chromatin in vivo. Levels of the chromo helicase protein CHD1 were reduced in Cdk9 knockdown chromosomes, suggesting that CHD1 is targeted to chromosomes through P-TEFb-dependent histone methylation. Dimethylation of histone H3 at lysine 36 was significantly reduced in knockdown larvae, implicating CTD phosphorylation in the regulation of this chromatin modification. Binding of the RNA Polymerase II elongation factor ELL was reduced in knockdown chromosomes, suggesting that ELL is recruited to active polymerase via CTD phosphorylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.