ArticleProceedings of the National Academy of Sciences of the United States of America2006
Prediction of protein orientation upon immobilization on biological and nonbiological surfaces.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 68 citations in OpenAlex.
- Coengineering Vapor-Deposited Polycationic Microenvironments and Immobilization Strategy to Enhance β‑Galactosidase Kinetics at Acidic pH.ACS omega · 2026Article
- Immobilization and docking studies of Carlsberg subtilisin for application in poultry industry.PloS one · 2023Article
- Article
- Differences in Charge Distribution inInternational journal of molecular sciences · 2022Article
- Article
- Achieving Controlled Biomolecule-Biomaterial Conjugation.Chemical reviews · 2018Review
- From Protein Features to Sensing Surfaces.Sensors (Basel, Switzerland) · 2018Review
- Mapping molecular binding by means of conformational dynamics measurements.RSC advances · 2018Article
- Structural insights into leishmanolysins encoded on chromosome 10 of Leishmania (Viannia) braziliensis.Memorias do Instituto Oswaldo Cruz · 2017Article
- Tunable control of antibody immobilization using electric field.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dynamics of lysozyme and its hydration water under an electric field.Journal of biological physics · 2014Article
- Lipid bilayer composition can influence the orientation of proteorhodopsin in artificial membranes.Biophysical journal · 2013Article
- Contribution of electrostatics to the binding of pancreatic-type ribonucleases to membranes.Biochemistry · 2013Article
- Three-dimensional stress field around a membrane protein: atomistic and coarse-grained simulation analysis of gramicidin A.Biophysical journal · 2013Article
- Modelling of lysozyme binding to a cation exchange surface at atomic detail: the role of flexibility.Biophysical journal · 2011Article
- Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor.Journal of proteomics · 2011Article
- Microfluidic systems for pathogen sensing: a review.Sensors (Basel, Switzerland) · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We report on a rapid simulation method for predicting protein orientation on a surface based on electrostatic interactions. New methods for predicting protein immobilization are needed because of the increasing use of biosensors and protein microarrays, two technologies that use protein immobilization onto a solid support, and because the orientation of an immobilized protein is important for its function. The proposed simulation model is based on the premise that the protein interacts with the electric field generated by the surface, and this interaction defines the orientation of attachment. Results of this model are in agreement with experimental observations of immobilization of mitochondrial creatine kinase and type I hexokinase on biological membranes. The advantages of our method are that it can be applied to any protein with a known structure; it does not require modeling of the surface at atomic resolution and can be run relatively quickly on readily available computing resources. Finally, we also propose an orientation of membrane-bound cytochrome c, a protein for which the membrane orientation has not been unequivocally determined.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.