ArticleMolecular and cellular biology2006
Upf1/Upf2 regulation of 3' untranslated region splice variants of AUF1 links nonsense-mediated and A+U-rich element-mediated mRNA decay.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Differential genetic analysis of ectrodactyly in a Fanconi anemia pedigree with FANCA mutations.MedScience · 2026Article
- Isoforms in action: The impact of transcript diversity in phenotype.Genetics and molecular biology · 2026Article
- Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages.Frontiers in immunology · 2022Article
- Pathogen-Associated Molecular Pattern-Triggered Immunity Involves Proteolytic Degradation of Core Nonsense-Mediated mRNA Decay Factors During the Early Defense Response.The Plant cell · 2020Article
- Genetic variants in mRNA untranslated regions.Wiley interdisciplinary reviews. RNA · 2018Review
- Murine knockin model for progranulin-deficient frontotemporal dementia with nonsense-mediated mRNA decay.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Auto- and cross-regulation of the hnRNPs D and DL.RNA (New York, N.Y.) · 2018Article
- Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered Nonsense Mediated mRNA Decay.PLoS genetics · 2016Article
- 5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion.EMBO molecular medicine · 2014Article
- AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation.Nucleic acids research · 2014Article
- RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing.The Journal of cell biology · 2011Article
- miR-375 inhibits differentiation of neurites by lowering HuD levels.Molecular and cellular biology · 2010Article
- RNA processing pathways in amyotrophic lateral sclerosis.Neurogenetics · 2010Review
- Circadian amplitude of cryptochrome 1 is modulated by mRNA stability regulation via cytoplasmic hnRNP D oscillation.Molecular and cellular biology · 2010Article
- Hypoxic inhibition of nonsense-mediated RNA decay regulates gene expression and the integrated stress response.Molecular and cellular biology · 2008Article
- Characterization of wound-responsive RNA-binding proteins and their splice variants in Arabidopsis.Plant molecular biology · 2008Article
- Analysis of turnover and translation regulatory RNA-binding protein expression through binding to cognate mRNAs.Molecular and cellular biology · 2007Article
- Post-transcriptional control of gene expression by AUF1: mechanisms, physiological targets, and regulation.Biochimica et biophysica actaReview
- The role of AUF1 in regulated mRNA decay.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
AUF1 is an RNA-binding protein that targets mRNAs containing A+U-rich elements (AREs) for rapid cytoplasmic turnover. Alternative pre-mRNA splicing produces five variants of AUF1 mRNA that differ in the composition of their 3'-untranslated regions (3'-UTRs). Previous work suggested that this heterogeneity in 3'-UTR sequence could regulate AUF1 expression by two potential mechanisms. First, AUF1 may regulate its own expression by binding to AREs in 3'-UTR splice variants that retain intron 9. The second potential mechanism, and the focus of this report, is regulation of a subset of 3'-UTR splice variants by the nonsense-mediated mRNA decay (NMD) pathway. Two of the five AUF1 mRNA 3'-UTR variants position the translational termination codon more than 50 nucleotides upstream of an exon-exon junction, creating a potential triggering signal for NMD in mammalian cells. Disruption of cellular NMD pathways by RNA interference-mediated knockdown of Upf1/Rent1 or Upf2/Rent2 or transfection of a dominant-negative Upf1 mutant specifically enhanced expression of these two candidate NMD substrate mRNAs in cells, involving stabilization of each transcript. Ribonucleoprotein immunoprecipitation experiments revealed that both Upf1 and Upf2 can associate with an NMD-sensitive AUF1 mRNA 3'-UTR variant in cells. Finally, quantitation of AUF1 mRNA 3'-UTR splice variants during murine embryonic development showed that the expression of NMD-sensitive AUF1 mRNAs is specifically enhanced as development proceeds, contributing to dynamic changes in AUF1 3'-UTR structures during embryogenesis. Together, these studies provide the first evidence of linkage between the nonsense- and ARE-mediated mRNA decay pathways, which may constitute a new mechanism regulating the expression of ARE-containing mRNAs.
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