ArticleMolecular and cellular biology2006
A gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed region.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Insights into FACT in Cancers with Targeted Therapeutic Implications.Molecular and cellular biology · 2026Review
- The Current Status of SSRP1 in Cancer: Tribulation and Road Ahead.Journal of healthcare engineering · 2022Review
- Regulatory networking of the three RNA polymerases helps the eukaryotic cells cope with environmental stress.Current genetics · 2021Review
- Comparative acetylome analysis reveals the potential roles of lysine acetylation for DON biosynthesis in Fusarium graminearum.BMC genomics · 2019Article
- FACT complex gene duplicates exhibit redundant and non-redundant functions in C. elegans.Developmental biology · 2018Article
- Acetylation-Dependent Recruitment of the FACT Complex and Its Role in Regulating Pol II Occupancy Genome-Wide inGenetics · 2018Article
- Level of FACT defines the transcriptional landscape and aggressive phenotype of breast cancer cells.Oncotarget · 2017Article
- Prognostic value of histone chaperone FACT subunits expression in breast cancer.Breast cancer (Dove Medical Press) · 2017Article
- Rat1p maintains RNA polymerase II CTD phosphorylation balance.RNA (New York, N.Y.) · 2014Article
- Complex mutual regulation of facilitates chromatin transcription (FACT) subunits on both mRNA and protein levels in human cells.Cell cycle (Georgetown, Tex.) · 2013Article
- Topoisomerase II is required for the production of long Pol II gene transcripts in yeast.Nucleic acids research · 2012Article
- The role of FACT in making and breaking nucleosomes.Biochimica et biophysica acta · 2012Article
- Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2.Biochemical and biophysical research communications · 2012Article
- The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation.Genetics · 2011Article
- Expression of FACT in mammalian tissues suggests its role in maintaining of undifferentiated state of cells.Oncotarget · 2011Article
- Curaxins: anticancer compounds that simultaneously suppress NF-κB and activate p53 by targeting FACT.Science translational medicine · 2011Article
- Yeast H2A.Z, FACT complex and RSC regulate transcription of tRNA gene through differential dynamics of flanking nucleosomes.Nucleic acids research · 2011Article
- Histone Chaperones Spt6 and FACT: Similarities and Differences in Modes of Action at Transcribed Genes.Genetics research international · 2011Article
- Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II.Genes & development · 2010Article
- The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The FACT complex stimulates transcription elongation on nucleosomal templates. In vivo experiments also involve FACT in the reassembly of nucleosomes traversed by RNA polymerase II. Since several features of chromatin organization vary throughout the genome, we wondered whether FACT is equally required for all genes. We show in this study that the in vivo depletion of Spt16, one of the subunits of Saccharomyces cerevisiae FACT, strongly affects transcription of three genes, GAL1, PHO5, and Kluyveromyces lactis LAC4, which exhibit positioned nucleosomes at their transcribed regions. In contrast, showing a random nucleosome structure, YAT1 and Escherichia coli lacZ are only mildly influenced by Spt16 depletion. We also show that the effect of Spt16 depletion on GAL1 expression is suppressed by a histone mutation and that the insertion of a GAL1 fragment, which allows the positioning of two nucleosomes, at the 5' end of YAT1 makes the resulting transcription unit sensitive to Spt16 depletion. These results indicate that FACT requirement for transcription depends on the chromatin organization of the 5' end of the transcribed region.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.