ArticleMolecular and cellular biology2006
Induction of gene silencing by hairpin RNA expression in Tetrahymena thermophila reveals a second small RNA pathway.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- The nucleosome assembly protein Nap1 regulates chromatin stability and nuclear division in Tetrahymena thermophila.Science China. Life sciences · 2026Article
- Bromodomain protein IBD1 bridges histone acetylation and H2A.Z deposition to fine-tune transcription.Nucleic acids research · 2026Article
- The Role of Membrane-Bound Extracellular Vesicles During Co-Stimulation and Conjugation in the CiliateMicroorganisms · 2025Article
- VPS8D, a CORVET subunit, is required to maintain the contractile vacuole complex inbioRxiv : the preprint server for biology · 2023Article
- Functional characterization of RebL1 highlights the evolutionary conservation of oncogenic activities of the RBBP4/7 orthologue in Tetrahymena thermophila.Nucleic acids research · 2021Article
- Article
- A distinct class of eukaryotic MT-A70 methyltransferases maintain symmetric DNA N6-adenine methylation at the ApT dinucleotides as an epigenetic mark associated with transcription.Nucleic acids research · 2019Article
- Condensins promote chromosome individualization and segregation during mitosis, meiosis, and amitosis inMolecular biology of the cell · 2018Article
- The piggyBac transposon-derived genes TPB1 and TPB6 mediate essential transposon-like excision during the developmental rearrangement of key genes in Tetrahymena thermophila.Genes & development · 2016Article
- Review
- Article
- A single cohesin complex performs mitotic and meiotic functions in the protist tetrahymena.PLoS genetics · 2013Article
- Strand-asymmetric endogenous Tetrahymena small RNA production requires a previously uncharacterized uridylyltransferase protein partner.RNA (New York, N.Y.) · 2012Article
- Tetrahymena thermophila JMJD3 homolog regulates H3K27 methylation and nuclear differentiation.Eukaryotic cell · 2012Article
- Functional specialization of Piwi proteins in Paramecium tetraurelia from post-transcriptional gene silencing to genome remodelling.Nucleic acids research · 2011Article
- The recombinases Rad51 and Dmc1 play distinct roles in DNA break repair and recombination partner choice in the meiosis of Tetrahymena.PLoS genetics · 2011Article
- RNA-Mediated Epigenetic Programming of Genome Rearrangements.Annual review of genomics and human genetics · 2011Review
- A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo.Genes & development · 2010Article
- A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila.Molecular biology of the cell · 2010Article
- Epigenetic inheritance in ciliates.Current opinion in microbiology · 2009Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 3 countries.
Funding
Abstract
Unlike in other eukaryotes, in which it causes gene silencing, RNA interference (RNAi) has been linked to programmed DNA deletion in the ciliate Tetrahymena thermophila. Here we have developed an efficient method to inducibly express double-stranded RNA hairpins and demonstrated that they cause gene silencing through targeted mRNA degradation in all phases of the life cycle, including growth, starvation, and mating. This technique offers a new tool for gene silencing in this model organism. Induction of RNA hairpins causes dramatic upregulation of Dicer and Argonaute family genes, revealing a system capable of rapidly responding to double-stranded RNA. These hairpins are processed into 23- to 24-nucleotide (nt) small RNAs, which are distinctly different from the 28- to 30-nt small RNAs known to be associated with DNA deletion. Thus, two different small RNA pathways appear to be responsible for gene silencing and DNA deletion. Surprisingly, expression of the RNA hairpin also causes targeted DNA deletion during conjugation, although at low efficiencies, which suggests a possible crossover of these two molecular paths.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.