ArticleMolecular and cellular biology2006
Nuclear localization signal and protein context both mediate importin alpha specificity of nuclear import substrates.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- CanFrontiers in cellular and infection microbiology · 2025Article
- An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single-cells.bioRxiv : the preprint server for biology · 2023Article
- Characterization of the nuclear import of the human CHD4-NuRD complex.Journal of cell science · 2023Article
- Nuclear Localization Signals for Optimization of Genetically Encoded Tools in Neurons.Frontiers in cell and developmental biology · 2022Article
- Article
- Differential Behaviours and Preferential Bindings of Influenza Nucleoproteins on Importins-α.Viruses · 2020Article
- Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection.Cell reports · 2020Article
- Karyopherin α-2 Mediates MDC1 Nuclear Import through a Functional Nuclear Localization Signal in the tBRCT Domain of MDC1.International journal of molecular sciences · 2020Article
- IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation.Journal of experimental & clinical cancer research : CR · 2019Article
- Importin α5 Regulates Anxiety through MeCP2 and Sphingosine Kinase 1.Cell reports · 2018Article
- Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Importin α1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons.PLoS pathogens · 2018Article
- Three-dimensional context rather than NLS amino acid sequence determines importin α subtype specificity for RCC1.Nature communications · 2017Article
- Nuclear import of the thyroid hormone receptor α1 is mediated by importin 7, importin β1, and adaptor importin α1.Molecular and cellular endocrinology · 2016Article
- Nuclear-cytoplasmatic shuttling of proteins in control of cellular oxygen sensing.Journal of molecular medicine (Berlin, Germany) · 2015Review
- Diversification of importin-α isoforms in cellular trafficking and disease states.The Biochemical journal · 2015Review
- Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7.Structure (London, England : 1993) · 2015Article
- Identification of importin α 7 specific transport cargoes using a proteomic screening approach.Molecular & cellular proteomics : MCP · 2014Article
- Conserved arginines of bovine adenovirus-3 33K protein are important for transportin-3 mediated transport and virus replication.PloS one · 2014Article
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5 authors.
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Abstract
The "classical" nuclear protein import pathway depends on importin alpha and importin beta. Importin alpha binds nuclear localization signal (NLS)-bearing proteins and functions as an adapter to access the importin beta-dependent import pathway. In humans, only one importin beta is known to interact with importin alpha, while six alpha importins have been described. Various experimental approaches provided evidence that several substrates are transported specifically by particular alpha importins. Whether the NLS is sufficient to mediate importin alpha specificity is unclear. To address this question, we exchanged the NLSs of two well-characterized import substrates, the seven-bladed propeller protein RCC1, preferentially transported into the nucleus by importin alpha3, and the less specifically imported substrate nucleoplasmin. In vitro binding studies and nuclear import assays revealed that both NLS and protein context contribute to the specificity of importin alpha binding and transport.
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