ArticleJournal of medical virology2006
Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness.
Article in Journal of medical virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers, 1 of them a synthesis that pooled it.
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Who cites it
96 citing papers in PubMed, 1 synthesis or guideline pooled it, 197 citations in OpenAlex.
- Human metapneumovirus in patients hospitalized with acute respiratory infections: A meta-analysis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2016Pooled it
- Clinical Presentation and Co-Detection of Respiratory Pathogens in Children Under 5 Years with Non-COVID-19 Bacterial and Viral Respiratory Tract Infections: A Prospective Study in Białystok, Poland (2021-2022).Medical science monitor : international medical journal of experimental and clinical research · 2023Article
- Role of Polymerase Chain Reaction-Based Diagnosis of Respiratory Viruses in Febrile Neutropenic Patients.Cureus · 2023Article
- Respiratory pathogens and clinical outcomes in children with an asthma exacerbation: A systematic review.Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2019Review
- Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia.Journal of virological methods · 2019Article
- Demographic and seasonal characteristics of respiratory pathogens in neonates and infants aged 0 to 12 months in the Central-East region of Tunisia.Journal of medical virology · 2019Article
- Simultaneous detection of 15 respiratory pathogens with a fluorescence probe melting curve analysis-based multiplex real-time PCR assay.International journal of molecular epidemiology and genetics · 2019Article
- Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction.Molecular medicine reports · 2018Article
- Mixed viral infections of the respiratory tract; an epidemiological study during consecutive winter seasons.Journal of medical virology · 2018Article
- Comparison of the prevalence of respiratory viruses in patients with acute respiratory infections at different hospital settings in North China, 2012-2015.BMC infectious diseases · 2018Article
- Respiratory Viruses in Febrile Neutropenic Patients with Respiratory Symptoms.Advanced biomedical research · 2018Article
- Detection of viral respiratory pathogens in mild and severe acute respiratory infections in Singapore.Scientific reports · 2017Article
- Respiratory viruses and influenza-like illness: Epidemiology and outcomes in children aged 6 months to 10 years in a multi-country population sample.The Journal of infection · 2017Article
- Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam.Wellcome open research · 2017Article
- Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia.PloS one · 2017Article
- Clinical Impact of Mixed Respiratory Viral Infection in Children with Adenoviral Infection.Infection & chemotherapy · 2016Article
- Bronchiolitis: Analysis of 10 consecutive epidemic seasons.Pediatric pulmonology · 2016Article
- Study and interest of cellular load in respiratory samples for the optimization of molecular virological diagnosis in clinical practice.BMC infectious diseases · 2016Article
- Article
- Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR.The Gazette of the Egyptian Paediatric Association · 2016Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The performances of four multiplex PCR (m-PCR) were compared to direct immunofluorescence assay (DFA) and HuH7 cell culture for the detection of viruses in 263 children admitted to hospital with an acute respiratory illness. One hundred fifty (57.6%) nasal aspirates were found DFA-positive; 188 (72.3%) were found positive by both DFA and HuH7 cell culture, and 242 (92%) were PCR-positive. The m-PCR detected 124 viruses which were not found by conventional methods: 68 rhinovirus, 17 human metapneumovirus, 15 respiratory syncytial virus (RSV), 8 parainfluenza virus (PIV), 5 coronavirus 229E, 3 OC43 and 3 NL63, 4 enterovirus, 2 influenza virus B and C virus. The m-PCR were more sensitive, had the advantages of a shorter delay in specific diagnosis, and a lower cost than DFA and culture. Using these m-PCR, the prevalence of each virus was compared between in-patient and out-patient groups of children attending the emergency unit of the hospital. Nasal aspirates from 411 (91.5%) children were found positive by the PCRs. RSV, rhinovirus, and influenza virus were the most frequent viruses detected in this population, representing 43.6%, 31.8%, and 8.8% of the virus found, respectively, followed by human metapneumovirus (4.4%), coronavirus (3.4%), parainfluenza virus (3.2%), adenovirus (2.3%), and enterovirus (2.1%). RSVs were detected more significantly in the in-patient group than in the out-patient group, and influenza viruses were detected more frequently in the out-patient group than in the in-patient group. Moreover, the use of m-PCR pointed out the frequency of rhinovirus and mixed viral detections in these patients. In conclusion, according to the requirements of speed and low cost of the methods, and to achieve the highest rate of detection of respiratory viruses, the combined use of DFA and m-PCR is today likely to be the best way to improve diagnosis of respiratory illnesses in children.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.