ArticleNucleic acids research2006
Transduction of human embryonic stem cells by ecotropic retroviral vectors.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation.EMBO reports · 2022Article
- Techniques of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Derivation.Archivum immunologiae et therapiae experimentalis · 2016Review
- Arylsulfatase A Overexpressing Human iPSC-derived Neural Cells Reduce CNS Sulfatide Storage in a Mouse Model of Metachromatic Leukodystrophy.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Article
- Small molecules enable highly efficient neuronal conversion of human fibroblasts.Nature methods · 2012Article
- Human embryonic stem cell-derived neurons establish region-specific, long-range projections in the adult brain.Cellular and molecular life sciences : CMLS · 2012Article
- Transduction of human cells with polymer-complexed ecotropic lentivirus for enhanced biosafety.Journal of visualized experiments : JoVE · 2011Article
- An XMRV derived retroviral vector as a tool for gene transfer.Virology journal · 2011Article
- Genetic manipulation of neural progenitors derived from human embryonic stem cells.Tissue engineering. Part A · 2009Article
- A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells.Stem cells (Dayton, Ohio) · 2009Article
- A rosette-type, self-renewing human ES cell-derived neural stem cell with potential for in vitro instruction and synaptic integration.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells.BMC biotechnology · 2008Article
- Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.Stem cells (Dayton, Ohio) · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The steadily increasing availability of human embryonic stem (hES) cell lines has created strong interest in applying available tools for gene transfer in murine cells to human systems. Here we present a method for the transduction of hES cells with ecotropic retroviral vectors. hES cells were transiently transfected with a construct carrying the murine retrovirus receptor mCAT1. Subsequently, the cells were exposed to replication-deficient Moloney murine leukemia virus (MoMuLV) derivatives or pseudotyped lentiviral vectors. With oncoretroviral vectors, this procedure yields overall transduction efficiencies of up to 20% and permits selection of permanently transduced clones with high frequency. Selected clones maintained expression of pluripotency-associated markers and exhibited multi-germ layer differentiation both in vitro and in vivo. HES cell-derived somatic cells including neural progeny maintained high levels of transgene expression. Lentiviral vectors pseudotyped with the MoMuLV envelope could be introduced in the same manner with efficiencies of up to 33%. Transgene expression of lentivirally transduced hES cells remained permanent after differentiation even without selection pressure. Bypassing the regulatory issues associated with the use of amphotropic retroviral systems and exploiting the large pool of existing murine vectors, this method provides a safe and versatile tool for gene transfer and lineage analysis in hES cells and their progeny.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.