ArticleApplied and environmental microbiology2006
Detection of human-derived fecal pollution in environmental waters by use of a PCR-based human polyomavirus assay.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 180 citations in OpenAlex.
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Infectious Pepper Mild Mottle Virus and Human Adenoviruses as Viral Indices in Sewage and Water Samples.Food and environmental virology · 2022Article
- Effectiveness of two wastewater disinfection strategies for the removal of fecal indicator bacteria, bacteriophage, and enteric viral pathogens concentrated using dead-end hollow fiber ultrafiltration (D-HFUF).The Science of the total environment · 2022Article
- Human Polyomaviruses (HPyV) in Wastewater and Environmental Samples from the Lisbon Metropolitan Area: Detection and Genetic Characterization of Viral Structural Protein-Coding Sequences.Pathogens (Basel, Switzerland) · 2021Article
- Evaluation of Low-Cost Phage-Based Microbial Source Tracking Tools for Elucidating Human Fecal Contamination Pathways in Kolkata, India.Frontiers in microbiology · 2021Article
- SV40 seroprevalence in two Latin American countries involved in field trials of candidate oral poliovaccines.The Journal of infection · 2019Article
- Relationships between Microbial Indicators and Pathogens in Recreational Water Settings.International journal of environmental research and public health · 2018Review
- Elucidating Waterborne Pathogen Presence and Aiding Source Apportionment in an Impaired Stream.Applied and environmental microbiology · 2018Article
- Presence of microbial and chemical source tracking markers in roof-harvested rainwater and catchment systems for the detection of fecal contamination.Environmental science and pollution research international · 2016Article
- Fecal Polyomavirus Excretion in Infancy.Journal of the Pediatric Infectious Diseases Society · 2016Article
- Distributions of Fecal Markers in Wastewater from Different Climatic Zones for Human Fecal Pollution Tracking in Australian Surface Waters.Applied and environmental microbiology · 2016Article
- Microbial quality and molecular identification of cultivable microorganisms isolated from an urban drinking water distribution system (Limassol, Cyprus).Environmental monitoring and assessment · 2015Article
- LA35 Poultry Fecal Marker Persistence Is Correlated with That of Indicators and Pathogens in Environmental Waters.Applied and environmental microbiology · 2015Article
- Comparison of concentration methods for quantitative detection of sewage-associated viral markers in environmental waters.Applied and environmental microbiology · 2015Article
- UVC Inactivation of dsDNA and ssRNA Viruses in Water: UV Fluences and a qPCR-Based Approach to Evaluate Decay on Viral Infectivity.Food and environmental virology · 2014Article
- Distribution of indigenous bacterial pathogens and potential pathogens associated with roof-harvested rainwater.Applied and environmental microbiology · 2014Article
- Faecal pollution loads in the wastewater effluents and receiving water bodies: a potential threat to the health of Sedibeng and Soshanguve communities, South Africa.Environmental science and pollution research international · 2014Article
- Coherence among different microbial source tracking markers in a small agricultural stream with or without livestock exclusion practices.Applied and environmental microbiology · 2013Article
- Ethnic differences in polyomavirus simian virus 40 seroprevalence among women in Houston, Texas.The Journal of infection · 2013Article
- Enterococci in the environment.Microbiology and molecular biology reviews : MMBR · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulatory agencies mandate the use of fecal coliforms, Escherichia coli or Enterococcus spp., as microbial indicators of recreational water quality. These indicators of fecal pollution do not identify the specific sources of pollution and at times underestimate health risks associated with recreational water use. This study proposes the use of human polyomaviruses (HPyVs), which are widespread among human populations, as indicators of human fecal pollution. A method was developed to concentrate and extract HPyV DNA from environmental water samples and then to amplify it by nested PCR. HPyVs were detected in as little as 1 microl of sewage and were not amplified from dairy cow or pig wastes. Environmental water samples were screened for the presence of HPyVs and two additional markers of human fecal pollution: the Enterococcus faecium esp gene and the 16S rRNA gene of human-associated Bacteroides. The presence of human-specific indicators of fecal pollution was compared to fecal coliform and Enterococcus concentrations. HPyVs were detected in 19 of 20 (95%) samples containing the E. faecium esp gene and Bacteroides human markers. Weak or no correlation was observed between the presence/absence of human-associated indicators and counts of indicator bacteria. The sensitivity, specificity, and correlation with other human-associated markers suggest that the HPyV assay could be a useful predictor of human fecal pollution in environmental waters and an important component of the microbial-source-tracking "toolbox."
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