ArticleJournal of bacteriology2006
Identification of non-dot/icm suppressors of the Legionella pneumophila DeltadotL lethality phenotype.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Membrane association and polar localization of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Adaptive β-lactam resistance from an inducible efflux pump that is post-translationally regulated by the DjlA co-chaperone.PLoS biology · 2023Article
- Evolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Species.Genome biology and evolution · 2019Article
- Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2019Review
- Legionella pneumophila strain associated with the first evidence of person-to-person transmission of Legionnaires' disease: a unique mosaic genetic backbone.Scientific reports · 2016Article
- Spatiotemporal regulation of a Legionella pneumophila T4SS substrate by the metaeffector SidJ.PLoS pathogens · 2015Article
- Article
- Evidence of the presence of a functional Dot/Icm type IV-B secretion system in the fish bacterial pathogen Piscirickettsia salmonis.PloS one · 2013Article
- Advances in gastric cancer prevention.World journal of clinical oncology · 2012Article
- The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates.PLoS pathogens · 2012Article
- Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system.Molecular microbiology · 2012Article
- Implication of proteins containing tetratricopeptide repeats in conditional virulence phenotypes of Legionella pneumophila.Journal of bacteriology · 2012Article
- Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host range expansion.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria.Frontiers in microbiology · 2011Article
- Global analysis of extracytoplasmic stress signaling in Escherichia coli.PLoS genetics · 2009Article
- DjlA, a membrane-anchored DnaJ-like protein, is required for cytotoxicity of clam pathogen Vibrio tapetis to hemocytes.Applied and environmental microbiology · 2008Article
- The response regulator CpxR directly regulates expression of several Legionella pneumophila icm/dot components as well as new translocated substrates.Journal of bacteriology · 2008Article
- CpxRA regulates mutualism and pathogenesis in Xenorhabdus nematophila.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Legionella pneumophila, a causative agent of bacterial pneumonia, survives inside phagocytic cells by avoiding rapid targeting to the lysosome. This bacterium utilizes a type IVB secretion system, encoded by the dot/icm genes, to replicate inside host cells. DotL, a critical component of the Dot/Icm secretion apparatus, functions as the type IV coupling protein. In contrast to most dot/icm genes, which are dispensable for growth on bacteriological media, dotL is required for the viability of wild-type L. pneumophila. Previously we reported that DeltadotL lethality could be suppressed by inactivation of the Dot/Icm complex via mutations in other dot/icm genes. Here we report the isolation of non-dot/icm suppressors of this phenotype. These DeltadotL suppressors include insertions that disrupt the function of the L. pneumophila homologs of cpxR, djlA, lysS, and two novel open reading frames, lpg0742 and lpg1594, that we have named ldsA and ldsB for lethality of DeltadotL suppressor. In addition to suppressing DeltadotL lethality, inactivation of these genes in a wild-type strain background causes a range of defects in L. pneumophila virulence traits, including intracellular growth, implicating these factors in the proper function of the Dot/Icm complex. Consistent with previous data showing a role for the cpx system in regulating expression of several dot/icm genes, the cpxR insertion mutant produced decreased levels of three Dot/Icm proteins, DotA, IcmV, and IcmW. The remaining four suppressors did not affect the steady-state levels of any Dot/Icm protein and are likely to represent the first identified factors necessary for assembly and/or activation of the Dot/Icm secretion complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.