ArticleBiophysical journal2006
Ca2+ selectivity of a chemically modified OmpF with reduced pore volume.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
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- Modeling the Device Behavior of Biological and Synthetic Nanopores with Reduced Models.Entropy (Basel, Switzerland) · 2020Article
- Electroosmosis Dominates Electrophoresis of Antibiotic Transport Across the Outer Membrane Porin F.Biophysical journal · 2020Article
- Interacting ions in biophysics: real is not ideal.Biophysical journal · 2013Review
- Modeling and simulation of ion channels.Chemical reviews · 2012Review
- Ionizable side chains at catalytic active sites of enzymes.European biophysics journal : EBJ · 2012Article
- Divalent Metal Ion Transport across Large Biological Ion Channels and Their Effect on Conductance and Selectivity.Biochemistry research international · 2012Article
- Selectivity sequences in a model calcium channel: role of electrostatic field strength.European biophysics journal : EBJ · 2011Article
- Simulations of calcium channel block by trivalent cations: Gd(3+) competes with permeant ions for the selectivity filter.Biochimica et biophysica acta · 2010Article
- The ionization state of D37 in E. coli porin OmpF and the nature of conductance fluctuations in D37 mutants.European biophysics journal : EBJ · 2010Article
- Sieving experiments and pore diameter: it's not a simple relationship.European biophysics journal : EBJ · 2010Article
- Energy variational analysis of ions in water and channels: Field theory for primitive models of complex ionic fluids.The Journal of chemical physics · 2010Article
- Protein structure and ionic selectivity in calcium channels: selectivity filter size, not shape, matters.Biochimica et biophysica acta · 2009Article
- Understanding ion conductance on a molecular level: an all-atom modeling of the bacterial porin OmpF.Biophysical journal · 2009Article
- Ions and inhibitors in the binding site of HIV protease: comparison of Monte Carlo simulations and the linearized Poisson-Boltzmann theory.Biophysical journal · 2009Article
- The anomalous mole fraction effect in calcium channels: a measure of preferential selectivity.Biophysical journal · 2008Article
- Bubbles, gating, and anesthetics in ion channels.Biophysical journal · 2008Article
- Engineering channels: atomic biology.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Energetics of divalent selectivity in a calcium channel: the ryanodine receptor case study.Biophysical journal · 2008Article
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Authors and funding
7 authors.
Funding
Abstract
We studied an E. coli OmpF mutant (LECE) containing both an EEEE-like locus, typical of Ca(2+) channels, and an accessible and reactive cysteine. After chemical modification with the cysteine-specific, negatively charged (-1e) reagents MTSES or glutathione, this LECE mutant was tested for Ca(2+) versus alkali metal selectivity. Selectivity was measured by conductance and zero-current potential. Conductance measurements showed that glutathione-modified LECE had reduced conductance at Ca(2+) mole fractions <10(-3). MTSES-modified LECE did not. Apparently, the LECE protein is (somehow) a better Ca(2+) chelator after modification with the larger glutathione. Zero-current potential measurements revealed a Ca(2+) versus monovalent cation selectivity that was highest in the presence of Li(+) and lowest in the presence of Cs(+). Our data clearly show that after the binding of Ca(2+) the LECE pore (even with the bulky glutathione present) is spacious enough to allow monovalent cations to pass. Theoretical computations based on density functional theory combined with Poisson-Nernst-Planck theory and a reduced pore model suggest a functional separation of ionic pathways in the pore, one that is specific for small and highly charged ions, and one that accepts preferentially large ions, such as Cs(+).
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